Registration Dossier

Toxicological information

Acute Toxicity: dermal

Currently viewing:

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1955
Report Date:
1955

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
0.5 ml per kg 2,6-di-tert-butylphenol were applied to the skin of rabbits and direct contact maintained for 24h.
GLP compliance:
no
Test type:
fixed dose procedure
Limit test:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
not specified

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Duration of exposure:
24 hours
Doses:
0.5 ml per kg
No. of animals per sex per dose:
10 rabbits (sex unknown)
Control animals:
no
Details on study design:
New Zealand rabbits ranging from 1.7 to 2.7 kg in body weight were used in these experiments. In preparation for the application of either of the compounds upon the skin of these animals, the hair was clipped as closely as possible from all areas of the trunk extending from the hips to the shoulders. A rubber sleeve, applied around the trunk in such manner as to fit snugly at its anterior and posterior edges but loosely in the intermediate area, covered the shorn skin of each rabbit. A measured amount of the specified compound was introduced beneath the sleeve and distributed in contact with as large an area of the bare skin as the dose would cover. The rubber sleeve was then covered completely with adhesive tape, and the animal was returned to its cage. After 24 hours had passed the sleeve was removed, and the skin was wiped as clean as possible with a dry towel.
Each of 10 rabbits was subjected in the manner described above, to contact of the skin with 2,6-di-tertiary butyl phenol in the dosage of 0.5 ml per kilogram.

Results and discussion

Preliminary study:
Each of 10 rabbits was subjected to contact of the skin with 2,6—di—tertiary butyl phenol in the dosage of 0.5 ml. per kilogram. These survived and exhibited no signs of illness and gained in weight at normal rates. Three animals developed a slight to moderate degree of erythema in the treated areas of the skin.
Effect levels
Sex:
not specified
Dose descriptor:
LD0
Effect level:
> 0.5 mL/kg bw
Remarks on result:
other: No signs of illness observed.
Mortality:
None
Clinical signs:
Three animals developed a slight to moderate degree of erythema in the treated areas of the skin.
Body weight:
Normal weight gain

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
Very low dermal toxicity and slightly irritating.
Executive summary:

Each of 10 rabbits was subjected to contact of the skin with 2,6-di-tertiary butyl phenol in the dosage of 0.5 ml. per kilogram. These survived and exhibited no signs of illness and gained in weight at normal rates. Three animals developed a slight to moderate degree of erythema in the treated areas of the skin.