Tris[2-chloro-1-(chloromethyl)ethyl] phosphate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation / corrosion, other

Remarks:

in vivo study performed in 1989

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study generated according to internationally accepted testing guidelines and according to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Details on test material:

- Name of test material (as cited in study report): Tolgard TDCP MK1
- Physical state: colourless liquid
- Storage condition of test material: in the dark under ambient conditions
- Analytical purity: not stated

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Cheshire Rabbit Farms Limited, Duddon Lodge, Tarporley,Cheshire.
- Housing: Housed individually in aluminium cages with grid floors beneath which were peat moss filled trays.
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: 8 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19 - 20 degrees C
- Humidity (%): 49%
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: clipped

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 ml
- Concentration (if solution): as from supplier, purity not stated
- Applied neat

Duration of treatment / exposure:

The test material (0.5ml) was applied to intact skin on each rabbit under a 2.5 x 2.5 cm patch of gauze. The patch was then covered with micropore tape and the trunk was loosely bound with Elastoplast Elastic bandage which remained in position for 4 hours.

Observation period:

1, 24, 48 and 72h after patch removal.

Number of animals:

3 animals (one male and 2 female)

Details on study design:

TEST SITE
- Area of exposure: 2.5 x 2.5 cm
- Type of wrap if used: patch of gauze, covered with micropore tape and loosely bound with elastoplast elastic bandage.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): wiped with water dampened tissues
- Time after start of exposure: 4 h

SCORING SYSTEM:
OECD recommended scoring system

Results and discussion

In vivo

Results

Irritation parameter:

erythema score

Basis:

mean

Time point:

other: 1 - 24h

Score:

>= 0 - < 2

Max. score:

2

Reversibility:

fully reversible within: 48h

Remarks on result:

other: Grade 2 erythema was noted in two animals after 1h and persisted to 24h in one animal. Grade 1 erythema was nothed in the third animal.

Irritant / corrosive response data:

Well defined erythema was recorded 1 h after patch removal in 2 rabbits and persisted to 24 h in one rabbit, the other improving to show only very slight erythema. Very slight erythema was recorded in the third rabbit, there was no oedema. All sites were normal 48 h after patch removal.

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information

Conclusions:

Tolgard TDCP MK1 is irritant to rabbit skin, but this is quickly reversible.