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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
4 Sep to 8 Sep 1989
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study OECD 203, GLP, no analytical monitoring. but results are consistent with QSAR prediction
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted 4 April 1984
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
no
Details on test solutions:
Dilution water: prepared according to test guideline following internal SOP's
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Danio rerio (Teleostei, Cyprinidae)
- Strain: Danio rerio (Teleostei, Cyprinidae) (HAMILTON-BUCHANAN)
- Source: Doller, Zierfischzüchterei, Finnentrop-Serkenrode, Germany
- Age at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): 2.0 ± 1.0 cm
- Weight at study initiation (mean and range, SD): mean weight: 0.27 ± 0.2 g
- Method of breeding: Laboratory bred
- Feeding during test: fish were not fed during the test (beginning 24 h prior to initiation)
- Food type: TetraMin® Hauptfutter
- Amount: no data
- Frequency: daily until 24 h before the test

ACCLIMATION
- Acclimation period: 12 days before start of the test
- Acclimation conditions (same as test or not): in dilution water of the same quality as used for the test
- Health during acclimation (any mortality observed): no data
Test type:
static
Limit test:
no
Total exposure duration:
96 h
Test temperature:
varied between 23 ± 1 °C
pH:
varied between 7.67 and 7.99
Dissolved oxygen:
in all test vessels between 7.38 mg/l and 8.10 mg/L
Nominal and measured concentrations:
Range finding test: 0 (blank), 0.1, 1.0, 10, 100 and 1000 mg/l.
Main test:0, 6.25, 12.5, 25, 50 and 100 mg/L (nominal test concentrations)
Details on test conditions:
Dilution water was prepared according to the test guideline (OECD 203) following internal SOP’s.

Test concentrations:
Aliquots of the stock solution were shaken for 24 h, then they were diluted with purified drinking water (dilution water).


Test priciples and procedure:

10 fish were used for each test concentration including controls.
Only fish in good health and free from any apparent malformation were used.


10-l glass aquaria were used as test vessels and filled with 5 l of test solution. The test animals were added to the previously prepared test solution; controls were kept in dilution water (blank).

The aquaria were slightly aerated via a glass capillary throughout the test.


The fish were exposed to various concentrations of the test substance for a period of 96 hours under static conditions. Visible abnormalities observed during the first 6 hours of the experiment were recorded. Mortalities and visual abnormalities were recorded at 24 h, 48 h, 72 h, and 96 h.

The test temperature was 23° C ± 1° C. The pH of the dilution water was 7.8 ± 0.2.
The light/dark rhythm was 14h/10h.

The pH and the oxygen concentrations of the control and of all the test concentrations were measured daily.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
15.95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The concentration-effect relationship and the LC50 were calculated with a computer program according to Lichtfield and Wilcoxon 1949 whereas the concentrations resulting in 0% and 100% mortality were derived directly from the test.

 Cumulative Mortality of Danio rerio (Zebra Fish)
                                                                                     Cumulative mortality of dead fish
 Nr   Test concentration (mg/l)  24h  48h  72h  96h
 0/0  blanc  0  0   0   0 
 1 6.25  0  0   0   0 
 2 12.5 (a)  0  0   1  1 
 3 25 (b)  5   10   10  10 
 4 50 (c)  10   10   10   10 
 5 100 (c)  10  10   10   10 

 (a) After 24 h of exposure the fish were calm and sluggish and swam with the backfin down. One dead after 2 days. Complete recovery of the surviving fish at day 4.

(b) The fish were instantly nervous and hectic and after 4 hours of incubation 70% were in side and/or back position. 50% dead after 1 day and 100% dead after 2 days.

(c) After 45 min the fish swam with the backfin down and died within 22 hours in vessel no. 4 and within 2 hours in vessel no. 5.

In the main test, in the 50 and 100 mg/l groups, after 45 minutes of exposure, the fish were swimming with the backfin down and all of the fish were dead within 2 (100 mg/l) or 22 hours (50 mg/l). The fish exposed to 25 mg/l were instantly nervous and hectic. After 4 hours of exposure, 70% were in the side or back position. After Day 1, 50% were dead and 100% were dead after 2 days. The fish exposed to 12.5 mg/l were calm and sluggish, swimming with their backfin down after 24 hours of exposure. One fish died after 48 hours of exposure. The remaining fish recovered by Day 4. There were no signs of toxicity observed in the fish exposed to 6.25 mg/l. 

Validity criteria fulfilled:
yes
Remarks:
-Criteria of OECD 203: Mortality of controls <1
Conclusions:
In a valid guideline study (OECD 203, static conditions), the 96 hr LC50 for Danio rerio (Teleostei, Cyprinidae) is 15.95 mg/L .
Executive summary:

In a 96-h acute toxicity study, Danio rerio (Teleostei, Cyprinidae) were exposed to Ethyleneglycol dimethacrylate

at 0 (control), 6.25, 12.5, 25.0,.50.0 and 100.0 mg/L nominal concentrations under static conditions. The 96-h LC50was 15.95 mg /L (nominal concentration). The LC0 and LC100 values, based on mortality effects, were 12.5 mg/L and 25 mg/L, respectively. The effect concentrations refer to the nominal.

 

This toxicity study is classified as acceptable and satisfies the guideline requirement for OECD 203, Danio rerio (Zebra fish) toxicity study.

 

Results Synopsis

 

Test organism size/age: mean weight: 0.27 ± 0.2 g, length: 2.0 ± 1.0 cm

Test Type: static

 

LC50: 15.95 mg/L                         95% C.I.: no data             

LC0: 6.25 mg/L   

LC100: 25 mg/L   

                   

Endpoint(s) Effected: mortality

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Description of key information

One acute freshwater study in fish is available for EGDMA, which is considered reliable without restriction (Klimisch score 1).

In a 96 h acute toxicity study, Danio rerio (Teleostei, Cyprinidae) were exposed to EGDMA nominal concentrations of 6.25, 12.5, 25, 50 and 100 mg/L under static conditions. 

The 48 h LC50 was 15.95 mg/L (based on mortality). The 48-h NOEC was 6.25 mg/L (based on mortality). 

Key value for chemical safety assessment

LC50 for freshwater fish:
15.95 mg/L

Additional information