Quaternary ammonium compounds, C20-22-alkyltrimethyl, chlorides

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990-08-2 to 1990-09-03

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: According OECD 209, non GLP, limited information on test substance

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

Instead of using 3,5-dichlorophenol as reference (acc. to OECD 209) potassium dichromate was used in a concentration to inhibit respiration of 50%.

Principles of method if other than guideline:

Instead of using 3,5-dichlorophenol as reference (acc. to OECD 209) potassium dichromate was used in a concentration to inhibit respiration of 50%.

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no furhter details are available

Analytical monitoring:

no

Details on sampling:

not applicable as nominal test concentrations are evaluated

Vehicle:

no

Details on test solutions:

no further details available

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Laboratory culture: activated sludge from municipal waste water treatment plant Hildesheim
- Method of cultivation:
- Preparation of inoculum for exposure: washed twice with dechlorinated, autoclaved tap water
- Pretreatment: none
- Initial biomass concentration: 1.251 g/L dry weight

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Post exposure observation period:

not applicable

Hardness:

not specified

Test temperature:

22°C

pH:

not specified

Dissolved oxygen:

not specified

Salinity:

not specified

Nominal and measured concentrations:

nominal: 10 - 18 - 32 - 58 - 100 - 180 mg/L

Details on test conditions:

TEST SYSTEM
TEST SYSTEM
- Test vessel:500 mL Erlenmeyer flasks
- Type (delete if not applicable): not specified
- Material, size, headspace, fill volume: 500mL
- Aeration: yes, permanently
- Type of flow-through (e.g. peristaltic or proportional diluter): not specified
- Renewal rate of test solution (frequency/flow rate):not specified
- No. of organisms per vessel:not specified
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic water acc. to OECD 209

OTHER TEST CONDITIONS
- Adjustment of pH: not specified
- Photoperiod: not specified
- Light intensity: not specified

Reference substance (positive control):

yes

Remarks:

kaliumdichromat

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

9.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: n.a.

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

16 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: n.a.

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

43 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: n.a.

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: n.a.

Details on results:

no further details available

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: 32 - 58 - 100 mg/ L

Reported statistics and error estimates:

no further details available

no further details available

Validity criteria fulfilled:

yes

Conclusions:

Test results are plausible and valid:
EC 10-Wert: 9.5 mg/L
EC 20-Wert: 16 mg/L
EC 50-Wert: 43 mg/L
EC 80-Wert: 120 mg/L

P 95 %: 11.26 < 43 < 164.17

Executive summary:

Test results are plausible and valid. Level of detail in the report reflect testing strategy and reporting of 1990.

Description of key information

Test results are plausible and valid:
EC 10-Wert: 9.5 mg/L
EC 20-Wert: 16 mg/L
EC 50-Wert: 43 mg/L
EC 80-Wert: 120 mg/L
P 95 %: 11.26 < 43 < 164.17

Key value for chemical safety assessment

EC50 for microorganisms:

43 mg/L

EC10 or NOEC for microorganisms:

9.5 mg/L