Propyl acetate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific principles (restriction: purity unknown)

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Range-Finding Toxicity Data: List VII

Author:

Smyth HF, Carpenter CP, Weil CS, Pozzani UC, Striegel JA, Nycum JS

Year:

1969

Bibliographic source:

Am. Ind. Hyg. Assoc. J. 30: 470-476

Reference 2

Reference Type:

study report

Title:

Unnamed

Year:

1961

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Groups of six rats were exposed for 4 hr to a dynamically generated vapor atmosphere containing n-propyl acetate test procedure according to Smyth et al. 1962 and older publications of this authors group (Range finding toxicity data lists; Assay of acute vapor toxicity)).

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Propyl acetate

EC Number:

203-686-1

EC Name:

Propyl acetate

Cas Number:

109-60-4

Molecular formula:

C5H10O2

IUPAC Name:

propyl acetate

Details on test material:

purity unknown

Test animals

Species:

rat

Strain:

other: Sherman

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: usually 100-150 g


ENVIRONMENTAL CONDITIONS
not reported

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other:

Details on inhalation exposure:

The exposure apparatus is described in sufficient detail in Smyth et al. 1949, "Assay for Acute Vapor Toxicity".

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

4000, 8000 and 16000 ppm = ca. 16.68, 33.36 and 66.72 mg/L

No. of animals per sex per dose:

6 animals per treatment of one sex (not reported in the publication)

Control animals:

not specified

Details on study design:

Animals were weighed prior to exposure and at the end of the post-exposure 14-day observation interval. Animals were observed for signs of toxicity during and after the exposure interval, and daily during the 14-day postexposure observation interval.
Autopsies are performed on all of the rats to ascertain that they did not die of extraneous infection. Gross pathologic change is recorded and liver, spleen, adrenal gland, kidney, and testis are taken for histopathological interpretation from 2 of the rats which died during the 14 day observation period and also from 2 that survived. The survivors are weighed and killed by transecting the spinal cord in the cervical region. An attempt is made to take the tissues for histopathological study from rats exposed to the concentration which killed either 2, 3, or 4 of the 6 exposed.

Statistics:

The LC50 value was calculated ex post with a probit analysis according to Finney.

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LC50

Effect level:

ca. 32 mg/L air

Exp. duration:

4 h

Mortality:

6/6 at 16000 ppm (ca. 66.72 mg/L)
4/6 at 8000 ppm (ca. 33.36 mg/L)
0/6 at 4000 ppm (ca. 16.68 mg/L)

Clinical signs:

other: Not reported in the publication.

Body weight:

Not reported in the publication.

Gross pathology:

Not reported in the publication.