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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with national standard method with acceptable restrictions (e.g. non-GLP, no analytical verification of test concentrations)
Qualifier:
according to guideline
Guideline:
other: German Industrial Standard DIN 38412, Part 15
GLP compliance:
no
Analytical monitoring:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The substance was added to the test water without any pretreatment. Subsequently the fish were placed into the aquaria.
Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name: golden orfe
- Source: Fischzucht Paul Eggers, Hohenwestedt, Germany
- Length at study initiation (length definition, mean, range and SD): body length: mean: 7.1 cm; range: 6.2-8.3 cm
- Weight at study initiation (mean and range, SD): mean: 4.6 g; range: 3.4-7.7 g
- Corpulence factor of batch: 1.3
- Feeding during test: no

ACCLIMATION
- Acclimation period: 7 weeks
- Acclimation conditions (same as test or not): not specified
- Type and amount of food: SSNIFF - growing feed F/B-50, ad libitum
- Health during acclimation (any mortality observed): 0 %

ADAPTATION
- Adaptation to test water and test temperature: 3 d
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
2.5 mmol/L
Test temperature:
23+-1 °C
pH:
about 7.9
Nominal and measured concentrations:
nominal test concentration: 500 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all-glass aquaria, 30 cm x 22 cm x 24 cm, 10 L
- Aeration: slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 4.6 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted freshwater
- guideline: German Industrial Standard DIN 38412, part 11, draft Sept. 1981
- preparation from fully demineralised tap water
- conductivity: max 10 µS
- resalting by addition of
- 294.0 mg/L CaCl2 * 2 H2O
- 123.3 mg/L MgSO4 * 7 H2O
- 64.8 mg/L NaHCO3
- 5.8 mg/L KCl
- continuous aeration with oil-free air
- Test water ready for use:
- Ca/Mg ratio: 4:1
- Na/K ratio: 10:1
- total hardness: 2.5 mmol/L
- acid capacity: 0.8 mmol/L
- pH about 7.9

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Mortality and symptoms: after 1, 4, 24, 48, 72 and 96 h
- pH, dissolved oxygen and temperature: after 1, 24, 48, 72 and 96 h

TEST CONCENTRATIONS
- Test concentration: 0 (control) and 500 mg/L
- Range finding study
- Results used to determine the conditions for the definitive study: LC50(96 h): > 500 mg/L
Reference substance (positive control):
yes
Remarks:
chloroacetamide
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Details on results:
- Mortality: no mortality observed
- Behavioural abnormalities: no symptoms detectable
- Mortality of control: 0 %
Results with reference substance (positive control):
- Results with reference substance valid? yes
- LC50(48 h): about 32 mg/L
Reported statistics and error estimates:
Reported LC50 with 1 % significance
Sublethal observations / clinical signs:
Table 1
- pH:
 Nominal concentration (mg/L)  beginning  24 h  48 h  72 h  96 h
0 (control)  7.7  7.8  7.9  8.0  8.0
 500  7.7  7.6  7.8  7.8  7.9

- Dissolved oxygen (mg/L):

 Nominal concentration (mg/L)  beginning  24 h  48 h  72 h  96 h
 0 (control)  7.3  6.7  7.2  7.1  7.2
 500  7.4  7.4  7.5  7.0  6.9

- Temperature (°C):

 Nominal concentration (mg/L)  beginning  24 h  48 h  72 h  96 h
 0 (control)  22  24  23  24  23
 500  23  24  23  24  24
Validity criteria fulfilled:
yes
Conclusions:
LC50 > 500 mg/L
Executive summary:

Trimethylamine hydrochloride, sol. 70 % (CAS 593 -81 -7) was tested in the golden orfe (Leuciscus idus) to evaluate its short-term toxicity potential to fish (BASF, 1987). The test procedure was conducted in accordance with national standard method (DIN 38412, Part 15) with acceptable restrictions (e.g. non-GLP, no analytical verification of test concentrations). The test substance (nominal 500 mg/L) was added to the test water without any pretreatment. Subsequently the fish (10 organisms per vessel) were placed into the aquaria. Mortality and symptoms were evaluated after 1, 4, 24, 48, 72 and 96 h. PH, dissolved oxygen and temperature were measured after 1, 24, 48, 72 and 96 h.

No mortality was observed and no behavioural abnormalities/ symptoms were detected. The LC50 value was determined to be > 500 mg/L.

Description of key information

Trimethylamine hydrochloride, sol. 70 % (CAS 593 -81 -7) was tested in the golden orfe (Leuciscus idus) to evaluate its short-term toxicity potential to fish (Key study 1, 1987). The test procedure was conducted in accordance with national standard method (DIN 38412, Part 15) with acceptable restrictions (e.g. non-GLP, no analytical verification of test concentrations). The test substance (nominal 500 mg/L) was added to the test water without any pretreatment. Subsequently the fish (10 organisms per vessel) were placed into the aquaria. Mortality and symptoms were evaluated after 1, 4, 24, 48, 72 and 96 h. PH, dissolved oxygen and temperature were measured after 1, 24, 48, 72 and 96 h.

No mortality was observed and no behavioural abnormalities/ symptoms were detected. The LC50 value was determined to be > 500 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
500 mg/L

Additional information