Tetrachloroethylene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP, near guideline study, published study report, fully adequate for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tetrachloroethylene

EC Number:

204-825-9

EC Name:

Tetrachloroethylene

Cas Number:

127-18-4

Molecular formula:

C2Cl4

IUPAC Name:

tetrachloroethene

Details on test material:

- Name of test material (as cited in study report): tetrachloroethylene- Analytical purity: 99.9%- Lot/batch No.: TA03116F-01 - Stability under test conditions: yes. Tetrachloroethylene was found to be stable for 2 weeks at 60ºC- Storage condition of test material: stored at 0ºC

Test animals

Species:

rat

Strain:

Fischer 344

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS- Source: Frederick Cancer Research Center- Age at study initiation: 5-7 weeks- Housing: stainless steel mesh, 1 animal per cage- Diet: ad libitum, except during inhalation exposure- Water: ad libitum, also during inhalation exposure- Acclimation period: 7 daysENVIRONMENTAL CONDITIONSdata not reported

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTIONTetrachloroethylene vapor generated by bubbling clean, dry air (-40ºC dewpoint) through all glass impingers that contained tetrachloroethylene; the different concentrations obtained by varying the amount of air that was passed through the test material.

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

2445, 3786, 4092, 4513 or 5163 ppm

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Animals were observed daily and weighed on days 0 and 15. Necropsy was performed on all animals.

Statistics:

not reported

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

other: lowest concentration causing mortality

Effect level:

3 786 ppm

Exp. duration:

4 h

Mortality:

All the rats that were exposed to 5163 ppm died before the end of the study, and deaths also occurred in all but the lowest dose groups.Survival: 5/5, 4/5, 3/5, 3/5, 0/5 (males) and 5/5, 1/5, 2/5, 2/5, 0/5 (females) in dose groups 2445, 3786, 4092, 4513, and 5163 ppm respectively.

Clinical signs:

other: Hypoactivity, ataxia, and anesthesia were observed in all dosed groups.

Body weight:

Mean body weight gain was not dose related.

Gross pathology:

No effects were reported.