Carbon disulphide

Toxicity to other aquatic organisms

Administrative data

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Toxicity and teratogenic effects of Carbon disulphide in frog embryo

Author:

Ghate HV

Year:

1985

Bibliographic source:

Rivista di Biologia Vol. 78

Reference 2

Reference Type:

secondary source

Title:

Carbon disulfide

Author:

GDCh-Advisory Committee on Existing Chemicals of Environmental Relevance (BUA)

Year:

1991

Bibliographic source:

BUA report 83

Materials and methods

Principles of method if other than guideline:

Effects of different concentrations of carbon disulphide on the embryonic development of frog investigated.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Carbon disulphide

EC Number:

200-843-6

EC Name:

Carbon disulphide

Cas Number:

75-15-0

Molecular formula:

CS2

IUPAC Name:

methanedithione

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

CS2 was dissolved in alcohol (1 mL CS2 in 50 mL alcohol). This solution was used to prepare various concentrations of CS2 in aged tap water. Controls received appropiate quantity of alcohol.

Test organisms

Test organisms (species):

Microhyla ornata

Details on test organisms:

Fresh, naturally fertilized eggs of the frog Microhyla ornata were obtained from a local, unpolluted pond. The jelly was removed with forceps and the eggs were transferred to aged tapwater. Eggs in early gastrulation (dorsal lip) were then used in experimentation as per the procedure described earlier (Ghate, 1980 & 1983 a). 50 Embryos per concentration expodes.

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Results and discussion

Details on results:

The embryonic development of frogs was disturbed at a concentration of 126 mg CS2/L, with no hatching taking place (100% mortality) .

Any other information on results incl. tables

4 µL CS2 / 100 mL aged tapwater: no mortality / Development normal

8 µL CS2 / 100 mL aged tapwater: no mortality / Hatching delayed by few hours / Tadpoles showed slightly abnormal notochord

12 µL CS2 / 100 mL aged tapwater: no mortality / Hatching delayed 50% failed to hatch even at 96 hrs. / All were oedematic with wavy notochord

16 µL CS2 / 100 mL aged tapwater: 90% mortality in 24 hrs. / All dead in 48 hrs.

20 µL CS2 / 100 mL aged tapwater: 100% mortality in 24 hrs.