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EC number: 921-024-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial pour density
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- Endpoint summary
- Stability
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- September - October 1982
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to national standards
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- read-across: supporting information
Reference
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- September - October 1982
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test procedure according to national standards
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- reference to same study
- Statistics:
- none
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other: There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other:
- Remarks:
- There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other:
- Remarks:
- There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other:
- Remarks:
- There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other:
- Remarks:
- There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other:
- Remarks:
- There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli, other: WP2, WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of E. coli strains either in the presence or absence of a rat liver microsomal activation system
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- There was no evidence of test compound precipitation in the assay system.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
The purpose of this study was to determine the mutagenicity of the test substance hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics. An Ames reverse mutation assay was done on S. typhimurium strains, TA 1535, TA 1537, TA 1538, TA 98 and TA 100, and E. coli strains WP2 uvr A and WP2, both with and without metabolic activation. Species were tested at concentrations ranging from 0.31 to 8000 ug/plate. The test plates were incubated for 48 -72 hrs, and then the number of colonies were counted. For all species, there was no significant increase in the number of revertants as compared to negative controls. Positive controls showed a significant increase in mutations. The test substance is not mutagenic. - Executive summary:
This data is being read across from the source study that tested Hydrocarbon C7-C9, n-alkanes, isoalkanes, cyclics based on analogue read across.
The purpose of this study was to determine the mutagenicity of the test substance hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics. An Ames reverse mutation assay was done on S. typhimurium strains, TA 1535, TA 1537, TA 1538, TA 98 and TA 100, and E. coli strains WP2 uvr A and WP2, both with and without metabolic activation. Species were tested at concentrations ranging from 0.31 to 8000 ug/plate. The test plates were incubated for 48 -72 hrs, and then the number of colonies were counted. For all species, there was no significant increase in the number of revertants as compared to negative controls. Positive controls showed a significant increase in mutations. The test substance is not mutagenic.
Table: Relative Mutation Rate – Bacterial Experiment I without Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
3.91 |
- |
- |
- |
- |
- |
- |
- |
7.81 |
- |
- |
- |
- |
- |
- |
- |
15.6 |
- |
- |
- |
- |
- |
- |
- |
31.3 |
- |
- |
- |
- |
- |
- |
- |
62.5 |
0.8 |
0.7 |
1.2 |
0.1 |
0.7 |
0.2 |
1.4 |
125 |
0.9 |
0.6 |
0.7 |
0 |
0.4 |
0 |
0.7 |
250 |
0.7 |
0.8 |
0.6 |
0 |
0 |
0 |
0.3 |
500 |
0.7 |
0.7 |
0.9 |
0 |
0 |
0 |
0.1 |
1000 |
0.8 |
0.5 |
0.9 |
0 |
0 |
0 |
0 |
2000 |
0.7 |
0.6 |
0.7 |
0 |
0 |
0 |
0 |
4000 |
0.8 |
0.6 |
0.6 |
0 |
0 |
0 |
0 |
8000 |
0.6 |
0.5 |
0.7 |
0 |
0 |
0 |
0 |
Sodium azide 2 µg |
- |
- |
89.8 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
1.0 |
0.7 |
0.9 |
4-nitroquinoline-N-oxide 3 µg |
39.8 |
3.2 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
1.8 |
- |
- |
- |
Table: Relative Mutation Rate – Bacterial Experiment I with Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
31.3 |
- |
- |
- |
- |
- |
- |
- |
62.5 |
0.7 |
1.3 |
1.4 |
0.3 |
1.5 |
0.7 |
1.0 |
125 |
0.8 |
1.0 |
1.1 |
0.2 |
1.3 |
1.3 |
0.8 |
250 |
0.9 |
1.0 |
0.8 |
0.6 |
1.5 |
1.2 |
1.0 |
500 |
0.7 |
0.8 |
1.0 |
0.2 |
1.0 |
0.7 |
1.3 |
1000 |
0.7 |
0.7 |
1.0 |
0.4 |
0.7 |
0.2 |
1.4 |
2000 |
1.1 |
0.9 |
1.1 |
0 |
0.4 |
0 |
1.2 |
4000 |
1.0 |
1.0 |
1.0 |
0 |
0.7 |
0 |
1.1 |
8000 |
0.8 |
1.0 |
0.5 |
0 |
0.7 |
0 |
1.1 |
Sodium azide 2 µg |
- |
- |
15.2 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
3.7 |
5.3 |
3.1 |
4-nitroquinoline-N-oxide 3 µg |
1.2 |
3.6 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
4.4 |
- |
- |
- |
Table: Relative Mutation Rate – Bacterial Experiment II without Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
3.91 |
- |
- |
- |
1.1 |
- |
0.8 |
- |
7.81 |
- |
- |
- |
0.6 |
- |
0.9 |
- |
15.6 |
- |
- |
- |
0.7 |
0.9 |
0.8 |
- |
31.3 |
- |
- |
- |
0.4 |
1.0 |
0.8 |
1.1 |
62.5 |
1.0 |
1.0 |
1.2 |
0.4 |
0.7 |
0.4 |
1.0 |
125 |
1.0 |
1.0 |
0.9 |
0 |
0.5 |
0 |
0.9 |
250 |
0.7 |
0.8 |
1.4 |
- |
0.2 |
- |
1.0 |
500 |
0.9 |
0.7 |
1.1 |
- |
0 |
- |
0.7 |
1000 |
1.0 |
0.8 |
0.9 |
- |
0 |
- |
- |
2000 |
0.7 |
0.9 |
0.7 |
- |
- |
- |
- |
4000 |
0.9 |
0.8 |
0.9 |
- |
- |
- |
- |
8000 |
0.8 |
0.7 |
0.9 |
- |
- |
- |
- |
Sodium azide 2 µg |
- |
- |
86.6 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
1.1 |
1.1 |
1.3 |
4-nitroquinoline-N-oxide 3 µg |
36.7 |
5.3 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
1.2 |
- |
- |
- |
Table: Relative Mutation Rate – Bacterial Experiment II with Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
31.3 |
- |
- |
- |
1.1 |
0.9 |
0.8 |
- |
62.5 |
0.9 |
0.9 |
0.7 |
1.2 |
1.1 |
1.0 |
1.1 |
125 |
1.1 |
1.0 |
1.2 |
1.1 |
1.1 |
0.9 |
1.0 |
250 |
1.4 |
0.8 |
0.8 |
1.4 |
1.1 |
0.8 |
1.1 |
500 |
1.2 |
1.0 |
0.9 |
1.0 |
1.1 |
0.8 |
1.2 |
1000 |
1.0 |
1.1 |
0.8 |
0.5 |
0.9 |
0.4 |
0.9 |
2000 |
0.8 |
1.0 |
1.1 |
0 |
0.9 |
0.1 |
0.9 |
4000 |
0.8 |
0.9 |
0.8 |
- |
0.5 |
- |
0.9 |
8000 |
1.0 |
0.7 |
0.7 |
- |
0.4 |
- |
0.9 |
Sodium azide 2 µg |
- |
- |
18.2 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
9.7 |
6.1 |
6.5 |
4-nitroquinoline-N-oxide 3 µg |
1.1 |
2.8 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
5.2 |
- |
- |
- |
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
- Reference Type:
- publication
- Title:
- The genetic toxicology of some hydrocarbon and oxygenated solvents
- Author:
- Brooks, T.M. et al.
- Year:
- 1 988
- Bibliographic source:
- Mutagenesis 3(3): 227-232
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- - limited documentation of cytotoxicity data
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics
- IUPAC Name:
- Hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics
- Details on test material:
- - Name of test material (as cited in study report): SBP 100/140 (Special Boiling Point Spirit 100/140)
- Substance type: clear, colourless liquid
- Physical state: liquid
- Analytical purity: 100% pure commercial product
- Composition of test material, percentage of components: Special Boiling Point Spirits, SBP 100/140 is a water-white mixture of paraffins and cyclo-paraffins [65% n and isoparaffins, and 35% naphthenes] in the C7-C9 range with 0.01% aromatic hydrocarbons.
- Lot/batch No.: ex Tank 77 P; Indent 9200/9515; Ref LONO42279
- Stability: verified by IR spectra on 14 July 1982 and 20 January 1983; no differences between two spectra
- Storage condition of test material: stored in the dark at ambient temperatures
- Other: Code Number: Q.5811; IP 446/82
- Source: Shell Nederland Raffinaderij B.V., Rotterdam
- test material received on 9 July 1982
Constituent 1
Method
- Target gene:
- S. typhimurium strains: his-operon
E. coli strains: tryptophan-operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98, TA 100
- Additional strain / cell type characteristics:
- not specified
- Species / strain / cell type:
- E. coli, other: WP2, WP2 uvr A
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat liver homogenate S9 activation, 10 % S9 liver homogenate from Aroclor treated rats
- Test concentrations with justification for top dose:
- TA 1538 (-S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
TA 1538 (-S) mix): 0, 15.62, 31.25, 62.5, 125, 250, 500, 1000; (+S9 mix): 0, 31.25, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
TA 1537 (-S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
TA 1537 (-S9 mix): 0, 3.9, 7.81, 15.62, 31.25, 62.5, 125; (+S9 mix): 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 µg/mL
TA 1535 (-S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
TA 100 (-S9 mix): 0, 0.31, 0.62, 1.25, 2.5, 5, 10; (+S9 mix): 0, 7.81, 15.62, 31.25, 62.5, 125, 250 µg/mL
TA 100 (-S9 mix): 0, 31.25, 62.5, 125, 250, 500; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
TA 100 (-S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
TA 98 (-S9 mix): 0, 0.31, 0.62, 1.25, 2.5, 5, 10; (+S9 mix): 0, 7.81, 15.62, 31.25, 62.5, 125, 250 µg/mL
TA 98 (-S9 mix): 0, 3.9, 7.81, 15.62, 31.25, 62.5, 125; (+S9 mix): 0, 31.25, 62.5, 125, 250, 500, 1000, 2000 µg/mL
TA 98 (-S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
EC WP2 (-S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL
EC WP2uvrA (-S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000; (+S9 mix): 0, 62.5, 125, 250, 500, 1000, 2000, 4000, 8000 µg/mL - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: Tween 80 and ethanol
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- other: TA 1538, TA 98, TA 100: benzo(a)pyrene; TA 1537: neutral red; TA 1535: Sodium azide; and E. coli strains: 4-nitroquinoline-N-oxide
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 30 min
- Incubation period: 48-72 hours
NUMBER OF REPLICATIONS: All test were carried out in triplicate.
Two replicate assays were carried out on different days to confirm reproducibility of results. - Statistics:
- none
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other: There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other: There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other: There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 97
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other: There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other: There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of S. typhimurium strains either in the presence or absence of a rat liver microsomal activation system.
- Cytotoxicity / choice of top concentrations:
- other: There was a differential toxicity of the test material to individual strains of Salmonella and the reduction of toxicity in the presence of an S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli, other: WP2, WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- Concentrations up to 8000 µg/mL in agar layer cultures did not increase the reverse mutation frequency of E. coli strains either in the presence or absence of a rat liver microsomal activation system
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- There was no evidence of test compound precipitation in the assay system.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table: Relative Mutation Rate – Bacterial Experiment I without Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
3.91 |
- |
- |
- |
- |
- |
- |
- |
7.81 |
- |
- |
- |
- |
- |
- |
- |
15.6 |
- |
- |
- |
- |
- |
- |
- |
31.3 |
- |
- |
- |
- |
- |
- |
- |
62.5 |
0.8 |
0.7 |
1.2 |
0.1 |
0.7 |
0.2 |
1.4 |
125 |
0.9 |
0.6 |
0.7 |
0 |
0.4 |
0 |
0.7 |
250 |
0.7 |
0.8 |
0.6 |
0 |
0 |
0 |
0.3 |
500 |
0.7 |
0.7 |
0.9 |
0 |
0 |
0 |
0.1 |
1000 |
0.8 |
0.5 |
0.9 |
0 |
0 |
0 |
0 |
2000 |
0.7 |
0.6 |
0.7 |
0 |
0 |
0 |
0 |
4000 |
0.8 |
0.6 |
0.6 |
0 |
0 |
0 |
0 |
8000 |
0.6 |
0.5 |
0.7 |
0 |
0 |
0 |
0 |
Sodium azide 2 µg |
- |
- |
89.8 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
1.0 |
0.7 |
0.9 |
4-nitroquinoline-N-oxide 3 µg |
39.8 |
3.2 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
1.8 |
- |
- |
- |
Table: Relative Mutation Rate – Bacterial Experiment I with Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
31.3 |
- |
- |
- |
- |
- |
- |
- |
62.5 |
0.7 |
1.3 |
1.4 |
0.3 |
1.5 |
0.7 |
1.0 |
125 |
0.8 |
1.0 |
1.1 |
0.2 |
1.3 |
1.3 |
0.8 |
250 |
0.9 |
1.0 |
0.8 |
0.6 |
1.5 |
1.2 |
1.0 |
500 |
0.7 |
0.8 |
1.0 |
0.2 |
1.0 |
0.7 |
1.3 |
1000 |
0.7 |
0.7 |
1.0 |
0.4 |
0.7 |
0.2 |
1.4 |
2000 |
1.1 |
0.9 |
1.1 |
0 |
0.4 |
0 |
1.2 |
4000 |
1.0 |
1.0 |
1.0 |
0 |
0.7 |
0 |
1.1 |
8000 |
0.8 |
1.0 |
0.5 |
0 |
0.7 |
0 |
1.1 |
Sodium azide 2 µg |
- |
- |
15.2 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
3.7 |
5.3 |
3.1 |
4-nitroquinoline-N-oxide 3 µg |
1.2 |
3.6 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
4.4 |
- |
- |
- |
Table: Relative Mutation Rate – Bacterial Experiment II without Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
3.91 |
- |
- |
- |
1.1 |
- |
0.8 |
- |
7.81 |
- |
- |
- |
0.6 |
- |
0.9 |
- |
15.6 |
- |
- |
- |
0.7 |
0.9 |
0.8 |
- |
31.3 |
- |
- |
- |
0.4 |
1.0 |
0.8 |
1.1 |
62.5 |
1.0 |
1.0 |
1.2 |
0.4 |
0.7 |
0.4 |
1.0 |
125 |
1.0 |
1.0 |
0.9 |
0 |
0.5 |
0 |
0.9 |
250 |
0.7 |
0.8 |
1.4 |
- |
0.2 |
- |
1.0 |
500 |
0.9 |
0.7 |
1.1 |
- |
0 |
- |
0.7 |
1000 |
1.0 |
0.8 |
0.9 |
- |
0 |
- |
- |
2000 |
0.7 |
0.9 |
0.7 |
- |
- |
- |
- |
4000 |
0.9 |
0.8 |
0.9 |
- |
- |
- |
- |
8000 |
0.8 |
0.7 |
0.9 |
- |
- |
- |
- |
Sodium azide 2 µg |
- |
- |
86.6 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
1.1 |
1.1 |
1.3 |
4-nitroquinoline-N-oxide 3 µg |
36.7 |
5.3 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
1.2 |
- |
- |
- |
Table: Relative Mutation Rate – Bacterial Experiment II with Metabolic Activation
Concentration |
E. coli WP2 |
E. coli WP2 uvr A |
S. typhimurium TA 1535 |
S. typhimurium TA 1537 |
S. typhimurium TA 1538 |
S. typhimurium TA 98 |
S. typhimurium TA 100 |
Without S9 |
|||||||
31.3 |
- |
- |
- |
1.1 |
0.9 |
0.8 |
- |
62.5 |
0.9 |
0.9 |
0.7 |
1.2 |
1.1 |
1.0 |
1.1 |
125 |
1.1 |
1.0 |
1.2 |
1.1 |
1.1 |
0.9 |
1.0 |
250 |
1.4 |
0.8 |
0.8 |
1.4 |
1.1 |
0.8 |
1.1 |
500 |
1.2 |
1.0 |
0.9 |
1.0 |
1.1 |
0.8 |
1.2 |
1000 |
1.0 |
1.1 |
0.8 |
0.5 |
0.9 |
0.4 |
0.9 |
2000 |
0.8 |
1.0 |
1.1 |
0 |
0.9 |
0.1 |
0.9 |
4000 |
0.8 |
0.9 |
0.8 |
- |
0.5 |
- |
0.9 |
8000 |
1.0 |
0.7 |
0.7 |
- |
0.4 |
- |
0.9 |
Sodium azide 2 µg |
- |
- |
18.2 |
- |
- |
- |
- |
Benzo(a)-pyrene 7 µg |
- |
- |
- |
- |
9.7 |
6.1 |
6.5 |
4-nitroquinoline-N-oxide 3 µg |
1.1 |
2.8 |
- |
- |
- |
- |
- |
Neutral red 7 µg |
- |
- |
- |
5.2 |
- |
- |
- |
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
The purpose of this study was to determine the mutagenicity of the test substance hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics. An Ames reverse mutation assay was done on S. typhimurium strains, TA 1535, TA 1537, TA 1538, TA 98 and TA 100, and E. coli strains WP2 uvr A and WP2, both with and without metabolic activation. Species were tested at concentrations ranging from 0.31 to 8000 ug/plate. The test plates were incubated for 48 -72 hrs, and then the number of colonies were counted. For all species, there was no significant increase in the number of revertants as compared to negative controls. Positive controls showed a significant increase in mutations. The test substance is not mutagenic. - Executive summary:
The purpose of this study was to determine the mutagenicity of the test substance hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics. An Ames reverse mutation assay was done on S. typhimurium strains, TA 1535, TA 1537, TA 1538, TA 98 and TA 100, and E. coli strains WP2 uvr A and WP2, both with and without metabolic activation. Species were tested at concentrations ranging from 0.31 to 8000 ug/plate. The test plates were incubated for 48 -72 hrs, and then the number of colonies were counted. For all species, there was no significant increase in the number of revertants as compared to negative controls. Positive controls showed a significant increase in mutations. The test substance is not mutagenic.
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