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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
02 Sep 1987 - 22 Sep 1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report date:
1988

Materials and methods

Principles of method if other than guideline:
Group of rats were exposed to test substance vapour for four hours and LC50 was determined.
GLP compliance:
not specified
Test type:
fixed concentration procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Hydrocarbons, C6-C7, n-alkanes, isoalkanes, cyclics, <5% n-hexane
EC Number:
921-024-6
Molecular formula:
none available - not a single isomer - see remarks
IUPAC Name:
Hydrocarbons, C6-C7, n-alkanes, isoalkanes, cyclics, <5% n-hexane
Details on test material:
- Name of test material (as cited in study report): SBP 60/95 LNH
- Physical state: liquid
- Analytical purity: 100% pure commercial product
- Storage condition of test material: at room temperature
- Other: colourless

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Interfauna UK Limited
- Age at study initiation: 6-8 weeks
- Weight at study initiation: 200 g
- Housing: polypropylene cages (38x56x18 cm)
- Diet: free access to a measured excess amount of food
- Water (ad libitum): tap water



ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21±0.8 - 25±1.3
- Humidity (%): 61±6.6

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: vapour generator plus exposure chamber
- Exposure chamber volume: 120 L
- Method of holding animals in test chamber: wire mesh compartments
- Source and rate of air: supply of clean dried air was connected to the generator and the supply pressure was adjusted to give a flow rate of 25 L per minute
- Temperature in air chamber: 25±0.33-25.9 °C


TEST ATMOSPHERE
- Brief description of analytical method used: analysed with a gas chromatograph. Samples were drawn through a gas absorption trap, containing 20 ml of acetone and cooled to -70 °C. Sampling rate was 2 litres/minute and the volume of air sample was measured with a wet-type gas meter.
- Samples taken from breathing zone: yes


Analytical verification of test atmosphere concentrations:
yes
Remarks:
Pye Unicam PU 4550 fitted with a flame ionisation detector. Pye Unicam PU 4700 autosampler.
Duration of exposure:
ca. 4 h
Concentrations:
25.2 mg/L air
No. of animals per sex per dose:
5
Control animals:
yes
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs at least twice daily, body weight daily, food and water consumption daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 25.2 mg/L air
Exp. duration:
4 h
Mortality:
There were no deaths during the study.
Clinical signs:
other: restless behavior and increase in respiration rate during the first 15 minutes; partial closing of the eyes
Body weight:
no effects
Gross pathology:
The lung weight to bodyweight ratio for all rats was considered to be within normal limits. No other macroscopic abnormalities were observed.
Other findings:
Food consumption was slightly reduced for 1 day following exposure. Water consumption was not affected by exposure.

Applicant's summary and conclusion

Interpretation of results:
study cannot be used for classification
Remarks:
Migrated information
Conclusions:
Under the conditions of the study a 4 hour LC50 >25.2 mg/L air was determined for the test substance, hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics < 5% hexane.
Executive summary:

Under the conditions of the study a 4 hour LC50 >25.2 mg/L air was determined for the test substance, hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics, < 5% n-hexane.