Hydrocarbons, C6-C7, n-alkanes, isoalkanes, cyclics, <5% n-hexane

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

02 Sep 1987 - 22 Sep 1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Principles of method if other than guideline:

Group of rats were exposed to test substance vapour for four hours and LC50 was determined.

GLP compliance:

not specified

Test type:

fixed concentration procedure

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics <5% n-hexane

IUPAC Name:

hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics <5% n-hexane

Details on test material:

- Name of test material (as cited in study report): SBP 60/95 LNH
- Physical state: liquid
- Analytical purity: 100% pure commercial product
- Storage condition of test material: at room temperature
- Other: colourless

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Interfauna UK Limited
- Age at study initiation: 6-8 weeks
- Weight at study initiation: 200 g
- Housing: polypropylene cages (38x56x18 cm)
- Diet: free access to a measured excess amount of food
- Water (ad libitum): tap water



ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21±0.8 - 25±1.3
- Humidity (%): 61±6.6

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: vapour generator plus exposure chamber
- Exposure chamber volume: 120 L
- Method of holding animals in test chamber: wire mesh compartments
- Source and rate of air: supply of clean dried air was connected to the generator and the supply pressure was adjusted to give a flow rate of 25 L per minute
- Temperature in air chamber: 25±0.33-25.9 °C


TEST ATMOSPHERE
- Brief description of analytical method used: analysed with a gas chromatograph. Samples were drawn through a gas absorption trap, containing 20 ml of acetone and cooled to -70 °C. Sampling rate was 2 litres/minute and the volume of air sample was measured with a wet-type gas meter.
- Samples taken from breathing zone: yes

Analytical verification of test atmosphere concentrations:

yes

Remarks:

Pye Unicam PU 4550 fitted with a flame ionisation detector. Pye Unicam PU 4700 autosampler.

Duration of exposure:

ca. 4 h

Concentrations:

25.2 mg/L air

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: clinical signs at least twice daily, body weight daily, food and water consumption daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, organ weights

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 25.2 mg/L air

Exp. duration:

4 h

Mortality:

There were no deaths during the study.

Clinical signs:

other: restless behavior and increase in respiration rate during the first 15 minutes; partial closing of the eyes

Body weight:

no effects

Gross pathology:

The lung weight to bodyweight ratio for all rats was considered to be within normal limits. No other macroscopic abnormalities were observed.

Other findings:

Food consumption was slightly reduced for 1 day following exposure. Water consumption was not affected by exposure.

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Remarks:

Migrated information

Conclusions:

Under the conditions of the study a 4 hour LC50 >25.2 mg/L air was determined for the test substance, hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics <5% hexane.

Executive summary:

Under the conditions of the study a 4 hour LC50 >25.2 mg/L air was determined for the test substance, hydrocarbons, C6-C7, n-alkanes, Isoalkanes, cyclics, <5% n-hexane.