Fatty acids C18 unsat, reaction products with triethylenetetramine

Long-term toxicity to aquatic invertebrates

Description of key information

One long term toxicity test with daphnia has been performed. The 21 d EC10  for reproduction is 270 µg/L based on the nomimal test substance concentration.  Corrected for active ingredient which is 94.4% the EC10 is reduced to 254.9 µg/L..

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

254.9 µg/L

Additional information

One long term toxicity test with daphnia has been performed. The 21 d EC10 for reproduction is 270 µg/L based on the nomimal test substance concentration. Corrected for active ingredient which is 94.4% the EC10 is reduced to 254.9 µg/L. The test was performed with river water as it is intended to be used in an evaluation of the environmental risks based on the Bulk approach where it is essential to show that the organisms were fully exposed to the nominal test concentration. During the test a decrease of the concentration is observed of about 70%. Imidazolines are susceptible to hydrolysis and the decrease observed could therefore be assigned to either hydrolysis or sorption or even both processes. Imidazolines will however only hydrolyze to an amidoamine which is relatively resistant to hydrolysis under ambient conditions. As this hydrolysis will also occur under environmental conditions and amidoamines have a comparable ecotoxicity as the imidazolines the Nominal concentration based on active ingredient is considered as the concentration to be used for the dose effect assesment as no significant adsorption to glassware was observed and the organisms were thus fully exposed the the nominal concentration based on active ingredient. Initially to the imidazoline which might have slowly changed into an amidoamine during the test. It is considered realistic to relate the observed effects to the nominal values based on active ingredient.