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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
11 Jan - 02 Feb 1990
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
according to guideline
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
not specified
according to guideline
other: Earthworm Acute Toxicity Tests based on the UBA-Guideline of March 1984
GLP compliance:
Analytical monitoring:
Details on sampling:
- Concentrations: 0 ppm, 30 ppm, 100 ppm, 300 ppm, 1000 ppm
Details on preparation and application of test substrate:
- Method of application to soil surface (if used): 5 g test substance were mixed with 95 g soil and dilutions of 3 : 10 and 1 : 10 were made. 10 g of each dilution were cooled by liquid air and together with deep frozen soil (150 g / vessel / concentration) homogenized. In a next step, the soil was mixed with the remaining amount of soil of 508 g.
Test organisms (species):
Eisenia fetida
Animal group:
Details on test organisms:
- Common name: earthworm
- Source: Dr. Edwards (EG-Ringtest 1983), than own breeding
- Age at test initiation (mean and range, SD): 2 month
- Weight at test initiation (mean and range, SD): 300 - 600 mg

- Acclimation period: 24 h
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): no mortality
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
Total exposure duration:
14 d
Test temperature:
20 +/- 2 °C
The water content was adjusted 7 days prior to the test start to 35.02% of the basic substrate (= 25.93% soil moisture)
Details on test conditions:
- Test container (material, size): glass vessels (1 - 2 L)
- Amount of soil or substrate: 658 g/vessel
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil): artificial soil according to the UBA guideline (March 1984)
Nominal and measured concentrations:
Nominal: 0, 30, 100, 300 and 1000 ppm
Reference substance (positive control):
14 d
Dose descriptor:
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: no mortalities at the highest concentration of 1000 ppm
Details on results:
- Mortality at end of exposure period: none
- Total mass of adults at beginning of test: since all worms survived at 1000 ppm and increase their body weight, only one replicate of the concentrations 30, 100 and 300 ppm was evaluated. 0 ppm: 9.48 g (start) / 9.51 g (end); 30 ppm: 2.5 g / 2.76 g; 100 ppm: 2.6 g / 2.64 g; 300 ppm: 2.33 g / 2.37 g; 1000 ppm: 9.28 g/ 9.75 g
- Changes in body weight of live adults (% of initial weight) at end of exposure period: 0 ppm: increase of body weight by 3.16%; 30 ppm: increase of body weight by 10.14%; 100 ppm: increase of body weight by 1.53%; 300 ppm: increase of body weight by 1.71%; 1000 ppm: increase of body weight by 5.06%.
- No. of offspring produced: not investigated
- No. of unhatched cocoons: not investigated
- Morphological abnormalities: not investigated
- Behavioural abnormalities: not investigated
- Other biological observations: none

Description of key information

LC50 (14d) > 1000 mg/kg soil dw. for Eisenia fetida 

Key value for chemical safety assessment

Additional information

The assessment of the acute toxicity to terrestrial macroorganisms is based on read-across to the analogue substance Alcohol ethoxylate C16-18, 10 EO (CAS 61791-28-4). The toxicity of Alcohols, C16-18, ethoxylated, 10 EO to Eisenia fetida was investigated in a study following OECD 207. Test concentrations of 30, 100, 300 and 1000 ppm were mixed with artificial soil. Earthworms of the species Eisenia fetida were exposed to the test concentrations of 14 days and the mortality of the earthworms was recorded. No mortality could be detected up to the highest test concentration of 1000 ppm. The determined EC50 (14 d) was > 1000 mg/kg soil dw.