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Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Description of key information

Daphnid EC50 (immibilization) is 2800 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
Effect concentration:
2 800 mg/L

Additional information

The acute toxicity of hexylene glycol was assessed in Daphnia magna in a freshwater, static test system as part of a series of tests conducted by Thurston et al. (1985). The 48-hour EC50 of hexylene glycol in Daphnia magna was determined to be 5410 mg/L.


Other acute toxicity tests conducted by Elnabarawy et al. (1986) were performed in Daphnia magnaDaphnia pulex and in Ceriodaphnia sp. under more or less similar conditions as above. The 48-hour EC50 of hexylene glycol in Daphnia pulex was determined to be 3300 mg/L, in Daphnia magna was 3200 mg/L and 2800 mg/L in Ceriodaphnia sp., based on nominal concentrations.


Tests were also performed on Tanytarsus dissimilis (Thuston et al., 1985) in a freshwater, static test system using closed beakers. Five to 7 dilutions of hexylene glycol were tested. The 96-hour LC50 of hexylene glycol was determined to be 4310 mg/L. The test performed in Orconectes immunis (crayfish) was conducted in a freshwater, flow-through system using 5 unspecified test concentrations and 1 control group. The 96-hour LC50 of hexylene glycol in Orconectes immunis was determined to be 16500 mg/L.


In addition, Linden et al. (1979 ) determined that the 96-hour IC50 of hexylene glycol was 7600 mg/L in adult Harpacticoid (Nitocra spinipes), based on nominal concentrations. The test was performed using a brackish water, static system in which 10 animals were exposed to each of 6 concentrations, with one control group in duplicate.