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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Additional information on environmental fate and behaviour

Administrative data

additional information on environmental fate and behaviour
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented scientifically sound study with sufficient documentation for proper assessment. No data on GLP and no guideline followed.

Data source

Reference Type:

Materials and methods

Principles of method if other than guideline:
In this study the biodegradation of 3-MCPD was inverstigated using whole-cell baker's yeast (Saccharomyces cerevisiae). Yeast cells were suspended in water, centrifuged and resuspended to form a yeast cream solution. The cream solution was placed in a 250 mL flask containing 3-MCPD. At different time points 3- MCPD was extracted from the reaction medium, analysed and quantified. The percentage degraded was plotted in time. To confirm that the biodegradation of 3-MCPD was due to an enzymatic reaction, the amount of chloride released i nthe reaction medium was compared to the amount of 3-MCPD transformed. For the latter a potentiometric tritration of chloride was performed. Throughout the study the effect of the pH, substrate stereospecificity, substrate concentration and carbon presence (glucose) on the biodegradation of 3-MCPD were also investigated.
GLP compliance:
not specified
Type of study / information:
Information on biodegradation other than in water, sediment and soil.

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): 3-Monochloro-1,2-propanediol (abbreviation used in report: 3-MCPD)
- Analytical purity: Analytical grade
- Source: Aldrich Chemical, Steinheim, Germany

Results and discussion

Any other information on results incl. tables

Effect of pH and substrate concentration:

Maximum biodegradation was seen at pH 8.2, where 45% of 3 -MCPD was converted after 48h, using an initial concentration of 27 mmol/L. For the groups at pH 7.0 and 6.2, this was only 35 and 12%, respectively. In contrast 60% of 3 -MCPD was converted at pH 8.2 after 48h when using a lower initial concencentration (i.e. 7.3 µmol/L).

Effect of carbon presence:

The addition of flucose as carbon source enhanced the 3 -MCPD degradation rate. Thus 68% substrate was transformed after 48h in the presence of glucose, while only 45% could be achieved in the abscence of glucose. Both trials were perfomed at pH 8.2 using an initial concentration of 27 mmol/L.

Effect of eneatiomers:

Results show that the (S)-enantiomer was degraded at a higher rate and percentage in comparison with the (R)-enantiomer. A maximum degradation of 85% was achieved with (S)-3 -MCPD, while only 60% degradation was observed for (R)-3 -MCPD

Chloride titration:

Results indicate that the degradation of 3 -MCPD is due to enzymatic reactions and not to other physicochemical phenomenons such as adsorption for instance.

The results of all assays showed good reproducibility (less than 2% standard deviation)

Applicant's summary and conclusion

The results in this study suggest that 3-chloro-1,2 propane diol is rapidly biodegradable by baker's yeat (S. cerevisiae). Furthermore the results showed that the pH, presence of a carbon source and stereoisomerism have an influence on the biodegradation rate of 3-MCPD. The maximum biodegradation was seen at a pH 8.2. A higher biodegradation rate was also absorved in the presence of a carbon source and for the (S)-enantiomer.