3-hydroxy-2-naphthoic acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

March 07-25, 1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study (OECD), GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

[adopted 1984]

Deviations:

yes

Remarks:

(test fish lenght, however EU guideline C.1 was fullfilled)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

[Council Directive 84/449/EEC, 1984]

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-hydroxy-2-naphthoic acid

EC Number:

202-180-8

EC Name:

3-hydroxy-2-naphthoic acid

Cas Number:

92-70-6

Molecular formula:

C11H8O3

IUPAC Name:

3-hydroxynaphthalene-2-carboxylic acid

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

68 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Symptoms: At the concentration of 10 mg/L after 24 h exposure increased activity was observed. At 50 and 71 mg/L the fish showed symptoms of intoxication (sticking out of gill covers; increased, intermittent respiration; tail-heavy swimming; loss of equilibrium; decreased activity; increased fright reaction). At 50 mg/L these symptoms have been observed only in the first 24 h. All fish in the 100 mg/L group died within the first 24 h.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

The mortality in the control did not exceed 10% at the end of the test; the dissolved oxygen concentration was at least 60% of the air saturation value throughout the test.

Conclusions:

The 96h-LC50 determined in this study was 68 mg/L

Executive summary:

In a 96-h acute toxicity study, zebra fish (Brachydanio rerio) were exposed to 3-Hydroxy-2-naphthoic acid at nominal concentrations of 0 (control), 10, 50, 71, and 100 mg/L under static conditions in accordance with OECD Guideline 203 and EU Method C.1 and in compliance with GLP. Reliability grade 1 was assigned. The 96-h LC50 was 68 mg/L. At the concentration of 10 mg/L increased activity was observed only after 24 h exposure. At 50 and 71 mg/L the fish showed several symptoms of intoxication. At 50 mg/L these symptoms have been observed only in the first 24 h. All fishes in the 100 mg/L group died within the first 24 h. No fish died in the control group.