Tris(2-butoxyethyl) phosphate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Principles of method if other than guideline:

24 hour exposure to intact or abraded skin.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tris(2-butoxyethyl) phosphate

EC Number:

201-122-9

EC Name:

Tris(2-butoxyethyl) phosphate

Cas Number:

78-51-3

Molecular formula:

C18H39O7P

IUPAC Name:

tris(2-butoxyethyl) phosphate

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Davidson's Hill Farm, Jamesburg, New Jersey
- Age at study initiation: no data
- Weight at study initiation: 2040-2815g
- Fasting period before study: no data
- Housing: individually in elevated metal cages
- Diet: Purina Lab Rabbit Chow ad libitum)
- Water: ad libitum
- Acclimation period: 1 week

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 hours

Doses:

200, 2000 and 5000

No. of animals per sex per dose:

4

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Mortality:

One female from 5000 mg/kg group was found dead on day 7

Clinical signs:

other: The only systemic clinical effects reported were slight to marked anorexia in all groups and eye discharge in a single mid-dose male. Local effects including erythema, oedema, blanching and epidermal scaling were reported.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Under the conditions of this study TBEP has a low acute dermal toxicity when tested on the rabbit.

Executive summary:

The test article, MCTR-16 -79 (tris(2-butoxyethyl) phosphate) was evaluated for acute dermal toxicity in groups of male and female New Zealand White rabbits. Half of the animals in each test group (4M + 4F) had an area of exposed skin (for subsequent dosing) abraded with minor incisions sufficiently deep to penetrate the stratum corneum but not deep enough to disturb the dermis or produce bleeding. The skin of the remaining rabbits was left intact.  The test substance was left in contact with the skin for 24-h under a non-absorbent binder. Dermal effects recorded following exposure were scored on days 1, 3, 7, 10 and 14 according to Draize. Surviving animals were killed on day 14 and a post-mortem examination was conducted.

One female animal of the 5000 mg/kg group was found dead on day 7. The only systemic clinical effects reported were slight to marked anorexia in all groups and eye discharge in a single mid-dose male. Local effects including erythema, oedema, blanching and epidermal scaling were reported.

According to the results of this study, the dermal LD50 is > 5000 mg/kg.