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Short-term toxicity to aquatic invertebrates

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Description of key information

'Yellow disazo condensation pigments' are with high probability acutely not harmful to aquatic invertebrates. No toxic effects occur at the range of the substance solubility. EC50 (48h) > 100 mg/L (nominal) for Daphnia magna was determined (OECD 202, EU Method C.2)

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The acute toxicity on aquatic invertebrates of the test substance was investigated with the waterflea Daphnia magna as test species according to OECD guideline 202 (CETA, 2011; CETA, 2001). Based on the low water solubility of the test item, a saturated stock solution was prepared and filtered through 0.45 µm filter. The test was conducted as limit test with a nominal concentration of 100 mg/L. After 48 hours of exposure an EC50 above 100 mg/L was determined.