Urea phosphate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

N/A to 1968-04-18

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Non-GLP study similar to OECD 423 with sufficient documentation on methods and results to evaluate data.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Deviations:

not specified

GLP compliance:

no

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Orthophosphoric acid

EC Number:

231-633-2

EC Name:

Orthophosphoric acid

Cas Number:

7664-38-2

IUPAC Name:

phosphoric acid

Details on test material:

- Name of test material (as cited in study report): thermal phosphoric acid
- Analytical purity: 75.4%

Test animals

Species:

rat

Strain:

other: SPF-Wister K

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 80-134 g
- Fasting period before study: 12 hrs
- Diet: After application rats received the standard-ALTROMIN R feed
- Water: tap water

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 10% aqueous solution
- Amount of vehicle (if gavage): 0.8 to 5.0- mL/100g-bw

Doses:

0.8, 1.25, 2.0, 3.2, and 5.0 mL per 100 g body weight

No. of animals per sex per dose:

10 female rats

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 7 days

Results and discussion

Effect levels

Sex:

female

Dose descriptor:

LD50

Effect level:

1.7 other: mL/100 g body weight

Mortality:

In the 0.8 mL/100 g-bw dose group there was no mortalities and in the 1.25 mL/100 g-bw group 2 of the 10 animals died. In the 2.00 mL/100 g-bw dose group 8 of the 10 animals died and in the 3.20 mL/100 g-bw and 5.0 mL/100 g-bw dose group all 10 of the animals died.

Clinical signs:

other: The animals died unbalanced, lying on tummy or flank, with breathing difficulties and cramps within 30 minutes to 24 hours after application.

Gross pathology:

The necropsy of the animals showed macroscopic strong chemical burns on the mucous membrane of the stomach.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The LD50 for a 10% solution of 75.4% thermal phosphoric acid in rats was determined to be 1.70 ml/100 g body weight (approximately 2600 mg/kg bw).