Registration Dossier

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Description of key information

48h EC50 100.9 mg/l for Daphnia magna

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
100.9 mg/L

Additional information

Three studies report on the toxicity of the test substance to Daphnia magna.

1. The toxicity of FeNaEDTA on Daphnia magna was tested in a 48 h static immobilisation test according to the OECD 202, EC method C.2 (92/69/EEC) and US EPA OPPTS 850.1010 (Borrmann 2007). The test was carried out under GLP. Test concentrations were measured and were within the range of 80% of the nominal concentration. The EC50 (mobility) was 100.9 mg/L (nominal) and the NOEC was 30 mg/L (reliability 1, Key study).

2. The acute toxicity (96h-LC50) of sodium ferric EDTA towards Daphnia magna was investigated in a multispecies test system according to EPA-600/3 -75 -009 and ASTM method E729 -80 (published by Ewell et al., 1986). The 96h-LC50 was 32 mg/l (nominal; published, reliability 2).

3. The acute toxicity of EDTA, Mn(II)+EDTA and Fe(III)+EDTA towards Daphnia magna was investigated according to ISO standard 6341 (published by Sorvari and Sillanpaa 1996, Reliability 2). The 24h-EC50 for Fe(III)+EDTA expressed as Fe(III) (= 17 mg/l) equals the value for Fe(III) when tested without EDTA (16 mg/l).

The 24h-EC50 for Fe(III)+EDTA expressed as EDTA is 89.3 mg/l. This is a lower value then the 610 mg/l for EDTA on its own. This may be due to the presence of free Fe present in the EDTA-Fe mixture that is absent in the test with EDTA on its own. The 24h-EC50 expressed as the test substance is 128.3 mg/l.

The key study with 48h-EC50 = 100.9 mg/l was selected because this was the only study carried out according to the guidelines and under GLP. The other studies were either longer (96h) or shorter and had other shortcomings and gaps of information.