3,7-dimethyloctan-3-ol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented study report which meets basic scientific principles

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Groups of 5 mice per sex and dose were treated simultaneously by intraperitoneal injection with preparations of the test substance in carboxymethylcellulose containing 2-3 drops of Cremophor EL. The concentrations of these preparations were usually adjusted to achieve comparable volumes (10 ml/kg bw).
Group-wise documentation of clinical signs was performed over the 7- to 14-day study period.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3,7-dimethyloctan-3-ol

EC Number:

201-133-9

EC Name:

3,7-dimethyloctan-3-ol

Cas Number:

78-69-3

Molecular formula:

C10H22O

IUPAC Name:

3,7-dimethyloctan-3-ol

Details on test material:

- Name of test material (as cited in study report): Tetrahydrolinalool (THL); Substance-ID: XXVI 402
- Physical state: fluid
- Analytical purity: > 98%
- Molecular weight (if other than submission substance): 158 g/mol
- Other: pH 7
- Solubility: water: no; alcohol: yes

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: average weight: male 30 g; female: 26 g
- Diet: Altromin R 1324, Altromin GmbH, Lage ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

CMC (carboxymethyl cellulose)

Details on exposure:

VEHICLE
- Concentration in vehicle: Pretest: 10 %, 46.4%, 100% Main Test: 3.16%, 4.64%, 6.81%, 10%
- Amount of vehicle: 10 ml/kg bw

MAXIMUM DOSE VOLUME APPLIED:10 ml/kg body weight

DOSAGE PREPARATION (if unusual): administered in 0,5 % in aquous
Carboxymethyl cellulose solution with 2 to 3 drops of Cremophor EL

Doses:

316, 464, 681, 1000 µl/kg bw:

No. of animals per sex per dose:

5 males and 5 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations of clinical symptoms: < 15 min, 15 min, 30 min, 1 h, 2 h, 4 h, 5 h; d1-d14 once per day on working days
- Necropsy of survivors performed: yes
- Frequency of weighing: day 3, 7 , 10

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 0.55 mL/kg bw

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

455 mg/kg bw

Remarks on result:

other: calculated with a density of 0.827 - 0.828 g/cm³

Clinical signs:

Dyspnoea, apathy, abdominal and lateral position, staggering, paresis of hindlimbs, partially atonia, partially sedate-like state with loss of cornea and pain reflex, partially rolling-and-stretching convulsions, partialy tonic and clonic spasms, exsiccosis, poor general condition, late cases of death, partially weight loss at the beginning of study

Body weight:

mean body weight:
dose 1000 µl/kg bw: male: day3: 26 g;
dose 1000 µl/kg bw: female: day3: 23 g; day 7: 25 g; day 10: 28 g;
dose 681 µl/kg bw: male: day3: 30 g, day 7: 34 g, day 10: 34 g;
dose 681 µl/kg bw: female: day3: 23 g; day 7: 27 g; day 10: 28 g;
dose 464 µl/kg bw: male: day3: 29 g, day 7: 31 g, day 10: 33 g;
dose 464 µl/kg bw: female: day3: 27 g; day 7: 29 g; day 10: 31 g;
dose 316 µl/kg bw: male: day3: 33 g, day 7: 34 g, day 10: 35 g;
dose 316 µl/kg bw: female: day3: 27 g; day 7: 28 g; day 10: 29 g;

Gross pathology:

In two mice (dose 1000 µl/kg bw) intraabdominal precipitation of substance was detected.
Other findings were not reported.

Any other information on results incl. tables

Mortality:

Pretest:

100 %: 5/5 female + 5/5 male animals after 30 min

46.4 %: 2/2 male animals after 15 min

10%: 2/2 male animals after 1h/3d

Main test:

dose (µl/kg bw)	concentration (%)	number of animals	mortality within
			1 h	24 h	48 h	7 d	14 d
1000	10	5 M	0/5	2/5	4/5	5/5	5/5
		5 F	0/5	0/5	1/5	3/5	4/5
681	6.81	5 M	0/5	2/5	2/5	4/5	4/5
		5 F	0/5	1/5	1/5	1/5	2/5
464	4.64	5 M	0/5	0/5	1/5	3/5	3/5
		5 F	0/5	1/5	1/5	1/5	1/5
316	3.16	5 M	0/5	0/5	0/5	0/5	0/5
		5 F	0/5	0/5	0/5	0/5	0/5