4-nitro-o-anisidine

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Main data provided, method acceptable

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1986

Materials and methods

Objective of study:

metabolism

Principles of method if other than guideline:

Rat were treated i.p with 20 mg test substance and urine was collected for 48 h. Urinary metabolites were determiend by TLC

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

4-nitro-o-anisidine

EC Number:

202-588-6

EC Name:

4-nitro-o-anisidine

Cas Number:

97-52-9

Molecular formula:

C7H8N2O3

IUPAC Name:

2-methoxy-4-nitroaniline

Constituent 2

Reference substance name:

PNOA

IUPAC Name:

PNOA

Radiolabelling:

not specified

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: tricaprylin

Details on exposure:

After single injection urine was collected individually for 48 h

Doses / concentrations

Remarks:

Doses / Concentrations:
20 mg/ animal
5mg/ml
no further data

No. of animals per sex per dose / concentration:

10

Control animals:

no

Details on dosing and sampling:

Urine was collected in metabolism cages at - 20 C from 0-24 and 24- 48 hours .
After thawing and filtration urines were extracted using ethyl acetate. at pH 6.5 (Extract A) and , after acidification with conc. HCl, at pH 3(Extract B). After 72 h at pH 3 at room temperature and neutralisation urines were again extracted (Extract C) and the process was repeated after repeated acidification to pH 3 (Extract D)
Extracts were then examined using TLC (UV/vis detection). Metabolites were identified by means of reference substances; Medium: Ethylacetate/diisopropylether =80/20 and Methyl-isobutyl-keton/Toluene = 20/80

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on excretion:

Besides the unchanged material the following metabolites were found:
1-Methoxy-2-N-acetylamino-5-aminobenzol (CAS-Nr. 5329-17-7),
2,5-Diacetylamino-1-methoxybenzol (CAS-Nr. 51837-64-0),
2-Amino-5-nitrophenol (CAS-Nr. 121-88-0) und
2 -N-Acetylamino-1-methoxy-5-nitrobenzol (CAS-Nr. 93-27-6)
in the urine

Metabolite characterisation studies

Metabolites identified:

yes