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EC number: 239-898-6 | CAS number: 15793-73-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 2012-06-14 to 2012-06-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- see Rationale and Justification for the Analogue Read-Across Approach used for the Registration of Pigment Orange 13, 34 and Pigment Red 38 (Chapter 13)
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was weighed out directly in an Erlenmeyer flask, filled up with 200 mL demieralised water and stirred overnight. Afterwards the pH of the additional concentration was adjusted to 7.5 +/- 0.5.
- Eluate: Test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL autoclaved tap water
- Differential loading: 560 - 750 - 1000 mg/L, additionally: 1000 mg/L (pH adjusted)
- Vehicle: none
- Replicates: triplicates
The test item was weighed out directly in an Erlenmeyer flask, filled up with 200 mL demineralized water and stirred overnight.
Afterwards the pH of the additional concentration was adjusted to 7.5 +/- 0.5.
Controls: Test medium without test or reference item.
- Replicates: Triplicates at the beginning of the test + triplicates at the end of the test - Test organisms (species):
- activated sludge
- Details on inoculum:
- - Preparation of inoculum for exposure: Non adapted activated sludge from the sewage plant at Hildesheim (Municipal sewage treatment plant of 31137 Hildesheim, Germany)
- Pretreatment: Washed twice with autoclaved tap water and adjusted to a dry weight concentration of 3 g/L +/- 10 %. The sludge was used within 24 h after stirring.
- Dry sludge concentration: 2.91 g/L corresponding to 1.46 g/L suspended solids in the test vessels - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Hardness:
- Not determined
- Test temperature:
- 21.2 °C
- pH:
- 7.45 (activated sludge)
7.44 (synthetic waste water) - Salinity:
- None, freshwater
- Nominal and measured concentrations:
- 560 - 750 - 1000 mg/L, additionally: 1000 mg/L (pH adjusted)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1000 mL Erlenmeyer flask, DIN 12380
- Aeration: Permanent
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3 at the beginning, 3 at the end of the test
- Composition of test medium:
250 mL inoculum
16 mL synthetic waste water
234 mL autoclaved tap water
Test or reference item concentration (directly weighed into each test vessel)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After 3 h incubation, the oxygen concentration of each control. reference item and test item replicate was determined by means of oximeter immediately after transferring the solution to the measuring cell and the oxygen depletion was recorded for at least 3 minutes on a flat bed recorder.
TEST CONCENTRATIONS
- Range finding study
Inhibition of Respiration (Preliminary Test)
Nominal Test Item Concentration
[mg/L] Pretreatment pH(test item in water) pH(test item in medium) Inhibition
[%]
10 Stirring overnight - - -6
100 Stirring overnight - - 2
1000 Stirring overnight - - 38
1000 Stirring overnight (6.00) 7.85* 7.42 1
1000 Stirring overnight 6.04 7.42 2
1000 Stirring overnight 6.02 7.48 11
1000 Stirring overnight (5.95) 7.12* 7.53 4
1000 Stirring overnight (5.96) 7.83* 7.60 -1
* adjusted with 1 M NaOH - Reference substance (positive control):
- yes
- Remarks:
- Copper (II) sulphate pentahydrate p.a.
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50: 91.2 mg/L - Reported statistics and error estimates:
- The NOEC was determined by calculation of statistical significance of the inhibition of respiration in comparison to the control. One Way Analysis of Variance (ANOVA) test was used for NOEC calculations. When running a One Way Analysis of Variance a Normality test and an Equal Variance test were done first. P-values for both Normality and Equal Variance test are 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) is a=0.05.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC of the test item is 1000 mg/L, the EC50 of the test item is > 1000 mg/L.
The test item is not toxic up to the concentration of 1000 mg/L to activated sludge of a municipal sewage treatment plant. - Executive summary:
A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out for the test item compliant to GLP (reliability category 1). Test system was activated sludge of the municipal treatment plant of 31137 Hildesheim, Germany. The test was carried out under static conditions with test item concentrations of 560 - 750 - 1000 mg/L in a geometrical series with a dilution factor of 1.33 and additionally with the highest test item concentration 1000 mg/L, adjusted to a pH value of 7.5 ± 0.5. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. The mean inhibition of respiration for the test item replicates ranged from -6 % to 1 %.
In order to check the activity of the test system and the test conditions a reference test was carried out with copper (II) sulphate pentahydrate as reference item and the reference toxicity was determined. The EC50-value for the reference item was 91.2 mg/L.
NOEC-Value of Test Item
Test Item Concentration
[mg/L]NOEC*
1000
*) no test item related statistically significant inhibition (p 0.05), ANOVA
The NOEC of the test item is 1000 mg/L, the EC50 of the test item is > 1000 mg/L.
The test item is not toxic up to the concentration of 1000 mg/L to activated sludge
of a municipal sewage treatment plant.
Reference
Oxygen Uptake Rates, Specific
Respiration Rates and Inhibition of the Respiration
of the Control, Test Item and Reference Item Concentrations
Test Item / |
|
pH* |
Oxygen Uptake Rate |
Specific Respiration Rate |
Inhibition |
Mean Inhibition |
|
Control |
1 |
25.2 |
17.3 |
– |
– |
||
2 |
27.6 |
18.9 |
|||||
3 |
26.4 |
18.1 |
|||||
4 |
23.2 |
15.9 |
|||||
5 |
23.6 |
16.2 |
|||||
6 |
23.6 |
16.2 |
|||||
Test Item |
560 |
1 |
27.6 |
-11 |
-5 |
||
2 |
26.0 |
-4 |
|||||
3 |
25.2 |
-1 |
|||||
750 |
1 |
26.4 |
-6 |
-2 |
|||
2 |
26.0 |
-4 |
|||||
3 |
23.6 |
5 |
|||||
1000 |
1 |
21.6 |
13 |
1 |
|||
2 |
24.8 |
0 |
|||||
3 |
27.2 |
-9 |
|||||
1000 |
1 |
7.74 |
28.0 |
-12 |
-6 |
||
2 |
7.80 |
25.6 |
-3 |
||||
3 |
7.57 |
26.0 |
-4 |
||||
Reference Item |
58 |
1 |
18.0 |
28 |
31 |
||
2 |
16.8 |
33 |
|||||
3 |
16.8 |
33 |
|||||
100 |
1 |
12.0 |
52 |
53 |
|||
2 |
12.0 |
52 |
|||||
3 |
11.2 |
55 |
|||||
180 |
1 |
5.6 |
78 |
79 |
|||
2 |
5.2 |
79 |
|||||
3 |
4.8 |
81 |
Repl. = Replicate
* adjusted with 0.1 M NaOH
Mean specific respiration rate of the control: 17.1 mg O2/g•h
Coefficient of variation of oxygen uptake rates of the control: 7.1 %
Suspended solids in the test vessel: 1.46 g/L
Description of key information
One toxicity to microorganism studies of reliability 1 for Pigment Red 38 is available.
Under the conditions of this test the NOEC of the submission substance after 3 hours was 1000 mg/L (based an nominal concentration) and is above the solubility limit of the test substance in the test water (2.2 μg/L).
For all three pigments of the Diazopyrazolone group consistently no aquatic toxicity has been found up to nominal concentrations well above the solubility limit. Therefore it is justified to use the data for all members of the group.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
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