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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1977
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1977
Report date:
1977

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
no guideline followed
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Zinc bromide
EC Number:
231-718-4
EC Name:
Zinc bromide
Cas Number:
7699-45-8
Molecular formula:
ZnBr2
IUPAC Name:
zinc bromide
Details on test material:
54% zinc bromide solution.
The test material was received from Great Lakes Chemical Corporation, West Lafayette, Indiana on July 22, 1977. It was identified as "Zinc Bromide Solution 54% (437-103)" and was received as a clear liquid.

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
Three male and 3 female New Zealand White rabbits (obtained from Kuiper's Rabbit Ranch, Gary, Indiana) were used for this study. The rabbits weighed from 2006 to 2168 grams at the beginning of the study. The rabbits were individually housed in metal metabolism cages in temperature and humidity controlled quarters . They were maintained in accordance with the recommendations contained in H.E.W. Publication No. 74-23 (N.I.H.) entitled "Guide for the Care and Use of Laboratory Animals". Water and Purina Rabbit Chow were available ad libitum.

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: The left eye served as the untreated control for each rabbit
Amount / concentration applied:
0.11 ml (100 mg zinc bromide)
Duration of treatment / exposure:
The rabbits received 0.11 milliliter by volume of the test material (100 mg of Zinc Bromide). The test material was placed into the cupped conjunctival sac of the right eye of each rabbit following which the eye lids were gently held together for one second. The left eye served as the untreated control for each rabbit. None of the rabbits received a washout.
Observation period (in vivo):
24, 48, 72 hours, 7, 14 days.
Number of animals or in vitro replicates:
3 males and 3 females

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 24 h
Score:
ca. 41.1
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 48 h
Score:
ca. 58.3
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 72 h
Score:
ca. 61.2
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 7 days
Score:
ca. 59.3
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 14 days
Score:
ca. 76.2
Irritant / corrosive response data:
24 h 48 h 72 h 7 days 14 days
cornea 22.5 38.5 38.5 37 55.5
Iris 4 6 6.5 8.5 8.5
Conjunctivae 14.6 13.8 16.2 13.8 12.2

Applicant's summary and conclusion

Interpretation of results:
highly irritating
Remarks:
Migrated information
Conclusions:
Based upon the data, the test material would be considered a primary eye irritant. The test material would b e further classified, based upon the scores obtained, as being extremely irritating to the eye when evaluated using the method described

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