Zinc bromide

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for data waiving:

other:

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1988

Materials and methods

Test guideline

Qualifier:

no guideline followed

Deviations:

not applicable

Principles of method if other than guideline:

The test material was administered intraperitoneally to mouse for 14 d to determine the LD50.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Zinc chloride

EC Number:

231-592-0

EC Name:

Zinc chloride

Cas Number:

7646-85-7

IUPAC Name:

zinc dichloride

Details on test material:

- Name of test material: Zinc chloride
- Other: Source - E Merck (Darmstadt, FRG)

Test animals

Species:

mouse

Strain:

Swiss

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Panlab (Barcelona, Spain)
- Age at study initiation: No data
- Weight at study initiation: 24-28 g
- Fasting period before study: No data
- Housing: No data
- Diet: Standard pellet diet (Panlab, Barcelona, Spain), ad libitum
- Water: Tap water, ad libitum
- Acclimation period: 7 d

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

not specified

Details on exposure:

DOSAGE PREPARATION: Solutions were given at pH between 6.0 and 7.0. Sodium bicarbonate was used to adjust the pH when necessary.

CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: A preliminary screening with small groups of three animals of each kind was carried out. The LD50 values were then calculated according to the Litchfield and Wilcoxon method.

Doses:

No data

No. of animals per sex per dose:

Preliminary screening: Three
Main study: Ten

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 d
- Frequency of observations and weighing: No data
- Necropsy of survivors performed: No
- Other examinations performed: Clinical signs, food and water consumption, weight gain

Statistics:

No data

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 91 mg/kg bw

95% CL:

57 - 146

Remarks on result:

other: Equivalent to 44 mg of Zn/kg

Mortality:

Mortality occurred mostly during the first 48 hr of test material administration. No deaths occurred after three days.

Clinical signs:

Miosis, conjunctivitis, necrosis in nose and exophthalmos were observed. These clinical signs appeared within the first 48 h and decreased/disappeared with time.
For details see Table 1 in "Remark on results including tables and figures" field

Body weight:

Slight weight loss

Gross pathology:

No data

Other findings:

No data

Any other information on results incl. tables

Table 1. Severity of physical and clinical signs in mice after zinc intoxication in a single dose

	Number of days after zinc administration
	1	2-3	4-7	8-14
Mortality rates on intraperitoneal administration	90%	10%	0%	0%
Conjunctivitis	None	+	+	None
Piloerection	None	+	+	+
Hemorrhages and hematomas in the tail	None	+	+++	+++
Degreased food and water consumption, weight loss	None	+	+	None

Mortality rates and physical and observational examination of rats are average for all zinc compounds.

+Light; ++Moderate; +++Severe

Applicant's summary and conclusion

Conclusions:

Under the test conditions, the acute intraperitoneal LD50 of the test material was calculated to be 91 mg/kg (equivalent to 44 mg of Zn/kg).

Executive summary:

A study was conducted to evaluate the acute intraperitoneal toxicity of the test material in Swiss albino mice.

Initially a preliminary screening with small groups of three mice of each kind was carried out and the LD50 values were calculated according to the Litchfield and Wilcoxon method. In the main study, the test material was administered in ten mice and observed for 14 d.

Conjunctivitis, piloerection, hemorrhages, hematomas in tail and decreased food and water consumption were observed in mice.

Under the test conditions, the acute intraperitoneal LD50 of the test material was calculated to be 91 mg/kg (equivalent to 44 mg of Zn/kg).