Registration Dossier
Registration Dossier
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EC number: 203-742-5 | CAS number: 110-16-7
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Maleic acid
- EC Number:
- 203-742-5
- EC Name:
- Maleic acid
- Cas Number:
- 110-16-7
- Molecular formula:
- C4H4O4
- IUPAC Name:
- but-2-enedioic acid
- Details on test material:
- - Name of test material (as cited in study report): Maleic acid
- Physical state: solid
- Analytical purity: 99.7%
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Community Sewerage Plant Bharath Electronics Limted, Vidyaranyapuram, Bangalore, INDIA
- Laboratory culture: The effluent was allowed to settle for one hour and decanted. The decanted effluent was then used in the test
- Method of cultivation: The bacterial population in the inoculum was determined as colony forming units (CFU/mL) by diluting the inoculum up to 10-4 dilution and then plating on nutrient agar plates - Duration of test (contact time):
- 29 d
Initial test substance concentration
- Initial conc.:
- 33.33 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: as described in the guideline
TEST SYSTEM
- Culturing apparatus: Conical flasks – 5 L capacity each fitted with an aeration tube reaching nearly to the bottom of the flask and an outlet
- Number of culture flasks/concentration: 4
- Method used to create aerobic conditions: continous gas flow
- Details of trap for CO2 and volatile organics if used: The amount of CO2 produced was calculated from the amount of base remaining in the absorption bottle. When 0.0125 M Ba(OH)2 was used as the absorbent, the amount remaining is assessed by titrating with 0.05 M HCl (thus, 50 mL HCl would be needed to titrate 100 mL Ba(OH)2).
SAMPLING
- Sampling frequency: every 3-4 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: yes
- Other: inhibition control
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- > 90
- Sampling time:
- 25 d
- Remarks on result:
- other: 10-day window reached
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
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