Registration Dossier
Registration Dossier
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EC number: 296-866-4 | CAS number: 93164-85-3
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06/1991-07/1991
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Amines, C20-22-alkyldimethyl
- EC Number:
- 296-866-4
- EC Name:
- Amines, C20-22-alkyldimethyl
- Cas Number:
- 93164-85-3
- Molecular formula:
- CnH2nN(CH3)2 n: from 18 to 22
- IUPAC Name:
- Amines, C20-22-alkyldimethyl
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Activated sludge dry weight: 2.37 g/L
Volume: 500 mL
Study design
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 18-22ºC
- Dissolved oxygen:
- 38.8 mg O2/L ·h
- Nominal and measured concentrations:
- Test-concentrations:100; 180; 320; 580; 1000 mg/L
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 1 000 mg/L
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Result:
EC20: 1000 mg/L
EC50: 1000 mg/L
EC80: 1000 mg/L - Executive summary:
Activated sludge respiration inhibition test
Test-concentrations:100; 180; 320; 580; 1000 mg/L Analysis of oxygen concentration: Continously with Oxygen-electrode (polarographic) and current-determination (Oxi 530, Fa. WTW) with temperature correction
Water: Synthetical waste water according to OECD 209
Inoculum: Activated sludge from a public waste water treatment plant of Hildesheim; was washed after decantation twice with de-chlored (autoclaved) tap water; dry-weight: 2.37 g/L
Reference substance: Coppersulfate-pentahydrate
Control: Inoculum without test substance Temperature 18-22 °C
Result:
EC20: 1000 mg/L
EC50: 1000 mg/L
EC80: 1000 mg/L
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