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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
06/1991-07/1991
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1984
Report date:
1984

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Amines, C20-22-alkyldimethyl
EC Number:
296-866-4
EC Name:
Amines, C20-22-alkyldimethyl
Cas Number:
93164-85-3
Molecular formula:
CnH2nN(CH3)2 n: from 18 to 22
IUPAC Name:
Amines, C20-22-alkyldimethyl

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge
Details on inoculum:
Activated sludge dry weight: 2.37 g/L
Volume: 500 mL

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
18-22ºC
Dissolved oxygen:
38.8 mg O2/L ·h
Nominal and measured concentrations:
Test-concentrations:100; 180; 320; 580; 1000 mg/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
1 000 mg/L
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
1 000 mg/L
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
1 000 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Result:
EC20: 1000 mg/L
EC50: 1000 mg/L
EC80: 1000 mg/L
Executive summary:

Activated sludge respiration inhibition test

 

Test-concentrations:100; 180; 320; 580; 1000 mg/L Analysis of oxygen concentration: Continously with Oxygen-electrode (polarographic) and current-determination (Oxi 530, Fa. WTW) with temperature correction

Water: Synthetical waste water according to OECD 209

Inoculum: Activated sludge from a public waste water treatment plant of Hildesheim; was washed after decantation twice with de-chlored (autoclaved) tap water; dry-weight: 2.37 g/L

 

Reference substance: Coppersulfate-pentahydrate

Control: Inoculum without test substance Temperature 18-22 °C

 

Result:

EC20: 1000 mg/L

EC50: 1000 mg/L

EC80: 1000 mg/L

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