Registration Dossier
Registration Dossier
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EC number: 291-103-1 | CAS number: 90341-71-2
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 > 500 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 511 mg/L
Additional information
The acute toxicity of Reactive Blue 220 was tested in Zebrafish (Danio rerio) for 96 hours at 500 mg/L. To determine the matter content, samples were taken at the beginning of the experiment and after 48 and 96 hours from the centre of the vessels.
The geometrical means of the concentration analyses resulted in 511 mg/L and therefore were above the requested 80% of the nominal concentration.
As a consequence of the dark blue colouration of the test water by the test substance, the observation of the behaviour of the fish was affected. However, as far as observation was possible, no behavioural changes were noted in the 500 mg/L test group.
No deaths were observed either in the control or the 500 mg/L test group. Hence, the LC50 after 48 and 96 hours is higher than 500 mg/L.
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