4,4'-bis(dimethylamino)-4''-(methylamino)trityl alcohol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Stock solution was prepared by dissolving 500 mg of the test substance in 1 liter of potable water (passed through reverse osmosis system) with 24 hrs of continuous stirring. After filteration water solubility obtained was 150 mg/L. From this stock solution, further test concentration was prepared for achieving test concentrations of 0.1 mg/L, 0.2 mg/L, 0.4 mg/L, 0.8 mg/L & 1.6 mg/L, respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Age of chemical: 3-4 months
- Length at study initiation (length definition, mean, range and SD): average 2.5 cm
- Weight at study initiation (mean and range, SD): 0.272 g (average)
- Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
- Duration of housing: 15 days
- Photoperiod: 12 hours light and 12 hours darkness
- Diet : Standard brand feed, once daily
- Acclimatization: seven days
- Feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

23°C

Dissolved oxygen:

7.5 mg/L

Nominal and measured concentrations:

Nominal concentrations: 0, 0.1, 0, 2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations)

Details on test conditions:

TEST SYSTEM
- Test vessel: Aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 4 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 12 hours light and 12-hour darkness

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Table 1: Mortality and symptoms:

NominalConcentration (mg/l)	Number of fish	Mortality (cumulated) after
		24h	48h	72h	96h
0 (control)	8	-	1	-	-
0.1	8	-	-	-	-
0.2	8	-	-	-	-
0.4	8	-	-	1	-
0.8	8	-	-	-	-
1.6	8	-	-	-	-

Visible Symptoms: Fishes were moving freely.

NominalConcentration(mg/l)	Number of fish	Symptoms after
		24h	48h	72h	96h
0 (control)	8	NS8	NS7	NS7	NS7
0.1	8	NS8	NS8	NS8	NS8
0.2	8	NS8	NS8	NS8	NS8
0.4	8	NS8	NS8	NS7	NS7
0.8	8	NS8	NS8	NS8	NS8
1.6	8	NS8	NS8	NS8	NS8

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

Table 2. pH and oxygen content:

NominalConcentration(mg/l)	pH readings after
	24h	48h	72h	96h
0 (control)	7.8	7.6	7.6	7.6
0.1	7.8	7.5	7.5	7.5
0.2	7.7	7.7	7.7	7.6
0.4	7.7	7.6	7.6	7.5
0.8	7.6	7.6	7.6	7.4
1.6	7.6	7.6	7.4	7.4

NominalConcentration(mg/l)	Oxygen content (mg/l) after
	24h	48h	72h	96h
0 (control)	7.2	7.0	6.9	6.9
0.1	7.1	7.0	6.9	6.8
0.2	7.1	6.9	6.8	6.8
0.4	7.2	7.0	6.9	6.8
0.8	7.3	7.2	7.1	6.7
1.6	7.3	7.2	7.0	6.5

Table 3. Test temperature

NominalConcentration(mg/l)	Temperature (°C) after
	24h	48h	72h	96h
0 (control)	21	22	23	23
0.1	21	22	23	23
0.2	21	22	23	23
0.4	21	22	23	23
0.8	21	22	23	23
1.6	21	22	23	23

Conclusions:

Based on nominal concentrations, the median lethal concentrations [LC50 (96 h)] for test chemical on Danio rerio (Zebra Fish) was determined to be > 1.6 mg/L.

Executive summary:

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test”. Zebra fish (Danio rerio) of average weight 0.272 g and average length of 2.5 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7.3, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance. Thus, on the basis of this limit test, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23°C, pH of 6.9, hardness of water 46.25 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the LC0 and median lethal concentration (LC50 (96 h)) value was determined to be 1.6 mg/l and > 1.6 mg/l, respectively.

Description of key information

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test” (Study report, 2015). Zebra fish (Danio rerio) of average weight 0.272 g and average length of 2.5 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7.3, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance. Thus, on the basis of this limit test, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23°C, pH of 6.9, hardness of water 46.25 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the LC0 and median lethal concentration (LC50 (96 h)) value was determined to be 1.6 mg/l and > 1.6 mg/l, respectively.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.6 mg/L

Additional information

Short term toxicity to fish was conducted for 96 hrs for assessing the effect of test chemical. The test was performed in accordance to OECD guideline No. 203 “Fish Acute Toxicity Test” (Study report, 2015). Zebra fish (Danio rerio) of average weight 0.272 g and average length of 2.5 cm was used as a test organism for the study. Test fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration. During the housing period, test fishes were fed once daily with standard brand fed. The test conditions during the housing of the test organisms were oxygen content of 7.4 mg/l, pH 7.3, water temperature 24°C and under a photoperiod of 12:12 hr light: dark conditions, respectively. The limit test (100 mg/l) was conducted for this substance. Thus, on the basis of this limit test, definitive test was performed at different test chemical nominal concentrations, i.e., at 0, 0.1, 0.2, 0.4, 0.8 and 1.6 mg/l (nominal concentrations), respectively. Total 8 fishes were exposed to test chemical in a 5 lit bowl aquaria containing 4 liters of potable water. The test vessels were placed in a room at a temperature of 23°C, pH of 6.9, hardness of water 46.25 mg of CaCO3 and under a photoperiod of 12:12 hr light: dark conditions, respectively. Aeration in test vessels was provided 1 day before the start of the experiment. Test fishes were moving slowly as compared to control. On the basis of effect of test chemical on mortality of the test organism, the LC0 and median lethal concentration (LC50 (96 h)) value was determined to be 1.6 mg/l and > 1.6 mg/l, respectively.