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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The effect of aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester towards the growth of activated sludge was investigated according to ISO 8192 -1986 (E) having an EC50(3 h) of 3110 mg/L (Bayer AG, 1998d).

Key value for chemical safety assessment

EC50 for microorganisms:
3 110 mg/L

Additional information

An adverse effect towards microorganisms and thus the cleaning capacity of sewage treatment plants is not expected based on the presented results.

Study was performed with aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester which is a structural analogue to aspartic acid, N,N'-(methylenedi-4,1-cyclohexanediyl)bis-, 1,1',4,4'-tetraethyl ester. Both substances are diethyl esters of aspartic acid linked to a dicyclohexylmethyldiamine moiety. The difference between these two substances is merely the presence of two methyl groups connected to the cyclohexane rings. This structural analogy was confirmed by the Member State responsible for the notification of both substances under the NONS regulation. The Member State decided that test results obtained for one substance can be transferred to the other substance and that testing of both substances is usually not required. This decision is in accordance with the grouping of substances and read-across approach in Annex XI, 1.5 of the REACH Regulation. 

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