Methyl chloroformate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: limited documentation and sone deviations to OECD 404. The study is however sufficient for evaluation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1975

Report date:

1975

Materials and methods

Principles of method if other than guideline:

2 rabbits were treated for 1, 5 and 15 minutes and for 20 hours using occlusive conditions.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroformate

EC Number:

201-187-3

EC Name:

Methyl chloroformate

Cas Number:

79-22-1

Molecular formula:

C2H3ClO2

IUPAC Name:

methyl chloroformate

Details on test material:

- Name of test material (as cited in study report): Chlorameisensäure methyl ester (chloroformic acid-methyl ester)
- Physical state: liquid
- no further data

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaucler, Offenbach, Germeny
- Weight at study initiation: 2.72 kg (male); 2.40 kg (female)
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
no data

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not required

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 ml

Duration of treatment / exposure:

1, 5 and 15 minutes and 20 hours

Observation period:

8 days

Number of animals:

4

Details on study design:

TEST SITE
- Area of exposure: 2.5 x 2.5 cm

REMOVAL OF TEST SUBSTANCE
- Washing (if done): 50 % Lutrol (mild detergent solution)
- Time after start of exposure: 1, 5 and 15 minutes and 20 hours

SCORING SYSTEM:
descriptive scoring system comparable to the Draize score

Results and discussion

In vivo

Results

Irritation parameter:

primary dermal irritation index (PDII)

Basis:

mean

Time point:

other: 24 h

Score:

6

Max. score:

8

Remarks on result:

other: necrocis

Irritant / corrosive response data:

full thicness necrosis was observed

Any other information on results incl. tables

Since irritation data are recommended for a 4 h skin exposition, results of the available 20 h and the 15 min exposition will serve as approximation.

Descriptive scores of the raw data have been converted to Draize numerical scores.Erythema scoring was at the border of the necrotic aerea.

Exposition:	20 h
Animal	Reading	Erythema	Edema	Comment
3	24 h	3	3	necrosis
4	24 h	3	3	necrosis
3	48 h	3	2	necrosis
4	48 h	3	3	necrosis
3	8 d	0	0	severe necrosis
4	8 d	0	0	severe necrosis
mean	24 - 48 h	3.0	2.75
Exposition:	15 min
Animal	Reading	Erythema	Edema	Comment
1	24 h	3	3	necrosis
2	24 h	2	4	necrosis
1	48 h	3	3	necrosis
2	48 h	2	3	necrosis
1	8 d	0	0	severe necrosis
2	8 d	0	0	severe necrosis
mean	24 - 72 h	2.5	3.25
Exposition:	5 min
Animal	Reading	Erythema	Edema	Comment
1	24 h	3	3	necrosis
2	24 h	3	3	necrosis
1	48 h	3	2	necrosis
2	48 h	3	3	necrosis
1	8 d	0	0	severe necrosis
2	8 d	0	0	severe necrosis
mean	24 - 72 h	3.0	2.75
Exposition:	1 min
Animal	Reading	Erythema	Edema	Comment
1	24 h	3	3	necrosis
2	24 h	3	3	necrosis
1	48 h	3	2	necrosis
2	48 h	3	3	necrosis
1	8 d	0	0	severe necrosis
2	8 d	0	0	severe necrosis
mean	24 - 72 h	3.0	2.75

Applicant's summary and conclusion

Interpretation of results:

corrosive

Remarks:

Migrated information