Methyl chloroformate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Necropsy revealed a significant inhalative coexposure causing severe, extensive hemorrhages of the lung in many animals. Inhalative coexposure may have caused additional deaths.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroformate

EC Number:

201-187-3

EC Name:

Methyl chloroformate

Cas Number:

79-22-1

Molecular formula:

C2H3ClO2

IUPAC Name:

methyl chloroformate

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: SPF breed: Hagemann, Extertal, Germany
- Weight at study initiation: males: 224 g; females: 183 g
- Fasting period before study:
- Diet: ad libitum; MRH concentrated feed of H . Eggersmann, Rinteln/Weser, Germany,
- Water: ad libitum

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: 13 .3 cm2; to the clipped skin of the back and flank
- Type of wrap if used: The treated area of skin was then covered with an inert foil, which was secured in position with adhesive tape .

REMOVAL OF TEST SUBSTANCE
- Washing with warm water and dried with cellulose
- Time after start of exposure: after 24 h

Doses:

2000; 1000 and 640 mg/kg .

No. of animals per sex per dose:

5 (640 and 2000 mg/kg); 6 (1000 mg/kg)

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

Probit analysis

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

894 mg/kg bw

95% CL:

693 - 1 161

Mortality:

After 14 days: 649 mg/kg: females 0/5, males 2/5; 1000 mg/kg: females 5/6, males 2/6; 2000 mg/kg: females 5/5, males 5/5

Clinical signs:

Dyspnea, apathy, staggering, cyanosis, tonic convulsions, spastic gait and piloerection
Signs of local irritation : After 24 hours, clear signs of primary irritation ; after 4 days, leathery necroses.

Body weight:

Mean body weight development of the surviving animals was positive.

Gross pathology:

Animals that died: Heart: acute dilatation on the right side; acute passive hyperemia;
lungs: severe, extensive hemorrhages (wet and sarcoid areas) in some cases
Sacrificed animals: Organ : no abnormalities detected

Any other information on results incl. tables

The product was applied once for 24 hours to the clipped skin of the back and flank (area of 13.3 cm2) unchanged in doses of 2,000 ; 1,000 and 640 mg/kg .The median lethal dose ( LD50) for males and females after 14 days was: 894 mg/kg (693 -1,161 mg/kg )