Methyl chloroformate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test conducted similar to OECD TG 401; no GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

: no control animal group

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl chloroformate

EC Number:

201-187-3

EC Name:

Methyl chloroformate

Cas Number:

79-22-1

Molecular formula:

C2H3ClO2

IUPAC Name:

methyl chloroformate

Details on test material:

- Name of test material (as cited in study report): Methylchloroformiat (methyl chloroformate)
- Physical state: liquid
- Analytical purity: 99 %

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: HAGEMANN
- Weight at study initiation: female (160 - 200 g); male (170 - 260 g)
- Fasting period before study: 15-20 h
- Diet (e.g. ad libitum): Herilan MRH-Haltung; H . Eggersmann KG

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

Concentration in vehicle: 43.00, 13.62, 9.28, 4.30, 2.94 % (w/v)

Doses:

2150, 681, 464, 215, 147 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

LD50-values were calculated using company software

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

313 mg/kg bw

95% CL:

229 - 427

Mortality:

147mg/kg bw: 1/10; 215mg/kg bw: 3 /10; 464mg/kg bw: 6/10; 681mg/kg bw: 10/10; 2150mg/kg bw: 10/10;

Clinical signs:

Dyspnea, apathy, staggering, tremors, piloerection, poor general state at all dose levels

Body weight:

All surviving animals gained weight

Gross pathology:

Animals that died: Heart: acute dilatation; acute passive hyperemia; stomach: hemorrhagic areas, in the forestomach in some animals ; scabbing
hemorrhagic gastritis in several animals; intestines: diffusely reddened mucosa in several animals; liver: peripheral acinar pattern sporadically
Sacrificed animals: Stomach: wall thickening at the tip of the forestomach; intraabdominal adhesion