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Long-term toxicity to fish

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Description of key information

The chronic toxicity of the read-across substance Guanidine Nitrate was tested In a 35-day fish early life stage test (FELS) against Fathead minnow. The duration of the test was  35 days. The NOEC  was 181mg/L and the lowest effect concentration was reported at 424mg/L (LOEC). 

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater fish:
181 mg/L

Additional information

The NOEC to LOEC of the read-across substance Guanidine Nitrate to Fathead minnow in the 35-day long-term toxicity tested in a fish early life stage test ranged from 181 to 424 mg/L. Mortality was the parameter where significant statistical effect were found. In contrast no statistical relavant effects could be observed for hatching success, fry defromities,or fry growth (length and weight).

Justification for read-across:

Guanidine hydrochloride and guanidine nitrate dissociate in aqueous media to yield the guanidine ion and the respective anion. Therefore it is reasonable to discuss the effects of the ions separately. The chloride ion is a naturally occurring essential ion in human beings with well-known metabolism and mechanisms of action as described in standard textbooks on pharmacology and physiology. As well it is found as salt in the Earth´s crust and is dissolved in seawater. Effects of guanidine hydrochloride are expected to be based primarily on the guanidine ion. The physiological processing of the guanidine ion is expected to be independent of the individual source. Therefore read-across from guanidine hydrochloride for effects of guanidine dissociated from guanidine nitrate is considered valid. This strategy is supported by a quite similar toxicological profile of both substances, as shown in acute toxicity, irritation, sensitization and genotoxic studies.

A more detailed justification is attached and outlined in CSR chapter 1.1.2 as well.