Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-808-0 | CAS number: 99-99-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- other: OECD SIDS
- Adequacy of study:
- other information
- Reliability:
- other: OECD SIDS
- Rationale for reliability incl. deficiencies:
- other: no reliability is given as this is a summary entry for the OECD SIDS
- Principles of method if other than guideline:
- OECD SIDS
- GLP compliance:
- not specified
- Executive summary:
OECD SIDS (2003):
In a semi-chronic test with Oryzias latipes a 28 d-NOEC of 0.8 mg/l for the endpoint effects mortality and swimming behaviour was determined. The results of the stability experiment performed before testing fish toxicity showed a decay of the test compound in the test medium of only 6 % after 8 days. Therefore nominal concentrations can be used (Canton et al., 1985). In another long-term study with Poecilia reticulata (semistatic exposure) a 28 d-NOEC of 10 mg/l concerning the endpoints mortality and swimming behaviour was found (Slooff and Canton, 1983). The same authors found in a chronic toxicity test to Oryzias latipes (semistatic exposure) a 40 d-NOEC of 32 mg/l concerning the endpoint hatching rate and a 40 d-NOEC of 1 mg/l concerning the endpoints mortality, growth and swimming behaviour. The reported values in this study are all nominal (Slooff and Canton, 1983). No explanation is given by the authors for the higher sensitivity of the endpoint mortality compared to hatching.
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- other: BUA report
- Adequacy of study:
- other information
- Reliability:
- other: BUA report
- Rationale for reliability incl. deficiencies:
- other: no reliability is given as this is a summary entry for the BUA report
- Principles of method if other than guideline:
- BUA report
- GLP compliance:
- not specified
- Executive summary:
BUA report (1989):
For the chronic effects of p-nitrotoluene to Poecilia reticulata Deneer et al. (1987) found a 14d-LC50 of 36.9 mg/L. In a 28d study on the effect of p-nitrotoluene with respect to mortality, growth and behavior of Poecilia reticulata, the no-effect concentration (NOLC, NOEC) was found to be 10 mg/L (Slooff & Canton, 1983). A 40d test on the effect of p-nitrotoluene on the eggs of Oryzias latipes showed a no-effect concentration (NOLC, NOEC) of 1 mg/L for mortality and larval behavior and 32 mg/L for the hatching rate (Slooff & Canton, 1983).
Canton et al. (1985) determined the following values as results of a 28d test with Oryzias latipes:
LC50 = 3.5 mg/L, EC50 (mortality and immobility) = 2.8 mg/L, NOEC (mortality and immobility) = 0.8 mg/L.
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- Test procedure in accordance with generally accepted scientific standards, but not described in sufficient detail
- Principles of method if other than guideline:
- other: Test similar to the method described by Canton and Slooff (1982) [Canton, JH and Slooff, W (1982): Substitutes for phosphate containing washing products: their toxicity and biodegradability in the aquatic environment. Chemosphere 11 (9): 891 - 907]
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Name of test material (as cited in publication): 4-Nitrotoluene
Analytical purity > 99.5 % Purity (Origin: Fluka) - Analytical monitoring:
- yes
- Details on sampling:
- no data
- Vehicle:
- not specified
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- - age of animals at start of test: 3-4 weeks
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 28 d
- Test temperature:
- 23 +/- 2 °C
- Details on test conditions:
- - 50 animals each test
- Fish were fed with Tetramine, Tetraphyll
- Photoperiod (day-/night rhythm)
- 3-times a week adjustment of the concentration
- Culturing and test medium: NaHCO3 100 mg/L, CaCl2*2H2O 200 mg/L, KHCO3 20 mg/L, MgSO4*7H2O 180 mg/L - Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.8 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and immobilisation
- Duration:
- 28 d
- Dose descriptor:
- LC50
- Effect conc.:
- 3.5 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- other: mortality and immobilisation
- Executive summary:
The toxicity of 4-Nitrotoluene to Oryzias latipes was evaluated in a 28 d study performed according to the method described by Canton and Sloof (1982). The 28d-NOEC value was determined to be 0.8 mg/L (Canton et al. 1985).
Referenceopen allclose all
OECD SIDS (2003):
In a semi-chronic test with Oryzias latipes a 28 d-NOEC of 0.8 mg/l for the endpoint effects mortality and swimming behaviour was determined. The results of the stability experiment performed before testing fish toxicity showed a decay of the test compound in the test medium of only 6 % after 8 days. Therefore nominal concentrations can be used (Canton et al., 1985). In another long-term study with Poecilia reticulata (semistatic exposure) a 28 d-NOEC of 10 mg/l concerning the endpoints mortality and swimming behaviour was found (Slooff and Canton, 1983). The same authors found in a chronic toxicity test to Oryzias latipes (semistatic exposure) a 40 d-NOEC of 32 mg/l concerning the endpoint hatching rate and a 40 d-NOEC of 1 mg/l concerning the endpoints mortality, growth and swimming behaviour. The reported values in this study are all nominal (Slooff and Canton, 1983). No explanation is given by the authors for the higher sensitivity of the endpoint mortality compared to hatching.
BUA report (1989):
For the chronic effects of p-nitrotoluene to Poecilia reticulata Deneer et al. (1987) found a 14d-LC50 of 36.9 mg/L. In a 28d study on the effect of p-nitrotoluene with respect to mortality, growth and behavior of Poecilia reticulata, the no-effect concentration (NOLC, NOEC) was found to be 10 mg/L (Slooff & Canton, 1983). A 40d test on the effect of p-nitrotoluene on the eggs of Oryzias latipes showed a no-effect concentration (NOLC, NOEC) of 1 mg/L for mortality and larval behavior and 32 mg/L for the hatching rate (Slooff & Canton, 1983).
Canton et al. (1985) determined the following values as results of a 28d test with Oryzias latipes:
LC50 = 3.5 mg/L, EC50 (mortality and immobility) = 2.8 mg/L, NOEC (mortality and immobility) = 0.8 mg/L.
Description of key information
For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".
OECD SIDS (2003):
In a semi-chronic test with Oryzias latipes a 28 d-NOEC of 0.8 mg/l for the endpoint effects mortality and swimming behaviour was determined. The results of the stability experiment performed before testing fish toxicity showed a decay of the test compound in the test medium of only 6 % after 8 days. Therefore nominal concentrations can be used (Canton et al., 1985). In another long-term study with Poecilia reticulata (semistatic exposure) a 28 d-NOEC of 10 mg/l concerning the endpoints mortality and swimming behaviour was found (Slooff and Canton, 1983). The same authors found in a chronic toxicity test to Oryzias latipes (semistatic exposure) a 40 d-NOEC of 32 mg/l concerning the endpoint hatching rate and a 40 d-NOEC of 1 mg/l concerning the endpoints mortality, growth and swimming behaviour. The reported values in this study are all nominal (Slooff and Canton, 1983). No explanation is given by the authors for the higher sensitivity of the endpoint mortality compared to hatching.
BUA report (1989)/Canton et al. (1985):
For the chronic effects of p-nitrotoluene to Poecilia reticulata Deneer et al. (1987) found a 14d-LC50 of 36.9 mg/L. In a 28d study on the effect of p-nitrotoluene with respect to mortality, growth and behavior of Poecilia reticulata, the no-effect concentration (NOLC, NOEC) was found to be 10 mg/L (Slooff & Canton, 1983). A 40d test on the effect of p-nitrotoluene on the eggs of Oryzias latipes showed a no-effect concentration (NOLC, NOEC) of 1 mg/L for mortality and larval behavior and 32 mg/L for the hatching rate (Slooff & Canton, 1983).
Canton et al. (1985) determined the following values as results of a 28d test with Oryzias latipes:
LC50 = 3.5 mg/L, EC50 (mortality and immobility) = 2.8 mg/L, NOEC (mortality and immobility) = 0.8 mg/L.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.