Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

Currently viewing:

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
Principles of method if other than guideline:
BASF Test: Sprague-Dawley rats, 10 per sex per group, were exposed sequentially to the vapours of the test substance for 4 hours at dose levels of 1.08, 0.28, 0.17, 0.12, 0.11, and 0.09 mg/l (2x). The vapours were generated by feeding a metered amount of test substance into an evaporator heated to 60°C and were then mixed with supply air. After exposure a 14-day observation period followed.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(dimethylamino)ethyl acrylate
EC Number:
219-460-0
EC Name:
2-(dimethylamino)ethyl acrylate
Cas Number:
2439-35-2
Molecular formula:
C7H13NO2
IUPAC Name:
2-(dimethylamino)ethyl prop-2-enoate
Details on test material:
- Name of test material (as cited in study report): Dimethylaminoethyl acrylate
- Physical state: clear liquid
- Analytical purity: > 90 %

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: MUS RATTUS, Brunnthal, FRG
- Weight at study initiation: 185 ± 15 g
- Diet: Herilan MRH (H . EGGERSMANN KG, Rinteln/Weser, FRG) ad libitum
- Water: Tap water ad libitum

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: air
Details on inhalation exposure:
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Wire mesh cages with 5 animals per cage were transfered to the whole-body exposure chamber made of glass and steel.
- Exposure chamber volume: 200 l
- Pressure in air chamber: negative pressure of 10 Pa
- Atmosphere generation: The vapours were generated by feeding a metered amount of test substance into an evaporator heated to 60°C and were then mixed with supply air.


TEST ATMOSPHERE
- Brief description of analytical method used: gas chromatograph (HP 5840 A)
- Samples taken from breathing zone: yes
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
Group 1: 1.08 mg/l; Group 2: 0.28 mg/l; Group 3: 0.17 mg/l; Group 4: 0.12 mg/l; Group 5: 0.11 mg/l; Group 6: 0.09 mg/l; Group 7: 0.09 mg/l
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: A check for dead or moribund animals and clinical symptoms was made daily. Bodyweights were determined before exposure, 7 days after exposure and at the and of the study.
- Necropsy of survivors performed: yes
Statistics:
Statistical analysis was performed according to Finney (Probitanalysis 1971, p. 1 - 150).

Results and discussion

Effect levelsopen allclose all
Sex:
male/female
Dose descriptor:
LC0
Effect level:
0.09 mg/L air (analytical)
Based on:
test mat.
Exp. duration:
4 h
Sex:
male/female
Dose descriptor:
LC50
Effect level:
0.22 mg/L air (analytical)
Based on:
test mat.
95% CL:
0.19 - 0.29
Exp. duration:
4 h
Sex:
male
Dose descriptor:
LC50
Effect level:
0.18 mg/L air (analytical)
Based on:
test mat.
95% CL:
0.15 - 0.25
Exp. duration:
4 h
Sex:
female
Dose descriptor:
LC50
Effect level:
0.27 mg/L air (analytical)
Based on:
test mat.
95% CL:
0.21 - 0.56
Exp. duration:
4 h
Mortality:
Animals died up to 5 days after exposure. Surviving animals of group 2 still showed symptoms after 14 days. The surviving animals of the other groups recovered after 5-7 days and showed no abnormal symptoms any more.
Clinical signs:
other: Group 1 to 3 showed aqueous to red nose and eye discharge, eyelid closure, dyspnoea, apathy, reduced pain reaction (1), cornea opacity (1,2), scrubby and clotted coat, lurching and high stepping gait, flatulence and abdominal position (1). Group 4 to 7 s
Body weight:
Female animals of group 2 and 4 and male animals of group 2 showed reduced body weight gain. Male animals of group 3, 4 and 5 showed reduced body weight gain after 7 days, which normalised after 14 days.
Gross pathology:
Animals that died: Heart: acute dilatation right, acute congested hyperemia; Lung: acute pulmonary emphysema, hyperemia with edema; Liver: sporadic marginally dilated periphery of lobules.
Sacrificed animals: Nothing abnormal detected.

Any other information on results incl. tables

Exposure levels and mortality:

Group

Analytical Concentration

(mg/l)

Mortality (dead rats/exposed rats)

Male

Female

Total

1

1.08

10/10

10/10

20/20

2

0.28

8/10

5/10

13/20

3

0.17

4/10

2/10

6/20

4

0.12

2/10

0/10

2/20

5

0.11

5/10

0/10

5/20

6

0.09

0/10

0/10

0/20

7

0.09

0/10

0/10

0/20

Applicant's summary and conclusion