Trichlorooctylstannane

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

08/1998

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study followed the OECD Guideline 203 (Fish, Acute Toxicity Test), but no GLP information provided.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: No. TX ME 549.3 of the Experimental Toxicology, Schering AG

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

mono-n-octyltin trichloride

IUPAC Name:

mono-n-octyltin trichloride

Constituent 2

Reference substance name:

26384

IUPAC Name:

26384

Details on test material:

- Name of test material (as cited in study report): mono-n-octyltin trichloride
- Purity test date: 98.86 %
- Lot/batch No.: Be 98/44
- Stability: at least until May 1999
- Formulation: ZK 26384 emulsified in tap water.
- Vapour pressure: 0.06 hPa at 25 ºC
- Safety precautions: routine
- Expiry date/stability: at least until May 99
- Storage conditions: dry, dark, cool
- Water solubility: 0.33 mg/L at 20 ºC
- Other: The tin-carbon bond of the octyltin compound is stable in aqueous solutions.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No information.

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

No information.

Test solutions

Vehicle:

not specified

Details on test solutions:

1.0045 g of liquid mono-n-octyltin trichloride was added to 4 L of tap water in a 10 L flask, which was then filled up to 10 L. This solution was stirred with a magnetic stir bar for approximately 67 hours. After this stirring period, the emulsion was filtered with the help of vacuum and thereafter filled into the aquarium. The filtrate was slightly opaque, but droplets of substance were not visible. The measured pH-value of this saturated solution was 8.0. For the control group and for dilution pure tap water was used. The water of the control group was stirred and filtered in the same way as for the test solution.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Zebra fish were bred in Netherlands (Charles River Aquatics); distributor: Charles River in Sulzfeld, Germany. Deviating from TXME 549.3 and in accordance with OECD No. 203 fish size was 2.0 cm ± 1.0 cm (the length of 5 randomly selected fish was measured).

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Mortalities and visual abnormalities were recorded at approximately 3, 6, 24, 48, 72 and 9 hours. Oxygen concentration, pH-value, and temperature were measured daily.

Test conditions

Hardness:

No information.

Test temperature:

Control:
0 hours: 21.6
24 hours: 20.9
48 hours: 20.9
72 hours: 20.9
96 hours: 21

Saturated Solution:
0 hours: 21.6
24 hours: 20.9
48 hours: 20.8
72 hours: 20.9
96 hours: 21

pH:

Control:
0 hours: 8.3
24 hours: 8.5
48 hours: 8.5
72 hours: 8.5
96 hours: 8.6

Saturated Solution:
0 hours: 8
24 hours: 8.3
48 hours: 8.5
72 hours: 8.5
96 hours: 8.6

Dissolved oxygen:

Control
0 hours: 8.6
24 hours:8.6
48 hours: 8.2
72 hours: 7.8
96 hours: 7.9

Saturated Solution:
0 hours: 8.3
24 hours: 8.3
48 hours: 8.0
72 hours: 7.7
96 hours: 7.7

Salinity:

No information.

Nominal and measured concentrations:

nominal loading of 100 mg/L.

Details on test conditions:

The fish were acclimatised for 20 days before the start of the test in tap water of the same quality used for the test. They were fed daily and the parameters oxygen content, pH-value, and temperature were measured each day. Normal tap water was used as dilution water, not polluted with harmful concentrations of chlorine, heavy metals and organochlorine pesticides based on quarterly measurements. The conductivity was 731 µS/cm, hardness (CaCO3) was 262.89 mg/L and the ratio Ca:Mg was 4:1.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.33 mg/L

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality was obsered in the saturated solution or the control during the whole expsoure time. All fish were free from abnormal behaviour. Due to the low observed toxicity of the filtered saturated solution of mono-n-octyltin trichloride a definative test was not performed an and LC50/96 hour value could not be determined.

Results with reference substance (positive control):

No information.

Reported statistics and error estimates:

No statistics were performed.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Nominal concentration of the unfiltered emulsion: 100 mg/L Concentration of the saturated filtered solution:< 1 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The 96-h LC50 of mono-n-octyltin trichloride to the Zebra fish (Danio rerio) was greater than 0.33 mg/L. No mortality or abnormal behaviour was observed in the test solution (or the control) during the whole exposure time. Mono-n-octyltin trichloride is not toxic to fish within the solubility limit (< 1 mg/L).

Executive summary:

Ten Zebra fish were exposed to a filtered saturated solution of mono-n-octyltin trichloride or to the dilution water (tap water) for a period of 96 hours (limit test). Mortalities and visible abnormalities were recorded at approximately 3, 6, 24, 48, 72 and 96 hours. The test temperature of the test and the control solutions was in the range of 20.8 to 21.6 °C; the pH-value was between 8.3 - 8.6 in the control and 8.0 - 8.6 in the test solution during the test period. The oxygen content was between 7.8 and 8.6 mg/L (88 - 98% of air saturation value) in the control, and 7.7 and 8.3 mg/L (86 - 94%) in the test solution. The acute 96-h LC50 of mono-n-octyltin trichloride to the Zebra fish (Danio rerio) was determined in a static aquatic system. Mortality and visible abnormalities of the test fish were recorded at 3, 6, 24, 48,72 and 96 h of exposure. No mortality and visible abnormalities of the test fish were observed in the test solution at saturation. It was determined that the 96-h LC50 of mono-n-octyltin trichloride to the Zebra fish (Danio rerio) was greater than 0.33 mg/L, the water solubility of the test substance under the test conditions. Mono-n-octyltin trichloride was not toxic to fish within the solubility limit (<1 mg/L).