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Environmental fate & pathways

Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2012-05-02 to 2012-05-03
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge
Radiolabelling:
no
Test temperature:
25 °C
Details on study design: HPLC method:
HPLC conditions:
- Column: LiChrospher 100 CN (5µm) 250x4, No: 933278
- Mobile Phase: methanol : water = 55 : 45 (v/v)
(pH value of ultrapure water: 6.4)
- Flow: 1 mL/min
- Injection volume: 10 µL
- Temperature: 25 °C
- Detector: UV 254 nm (Reference Items)
- Detector: UV 220 nm (Test Item)
Analytical monitoring:
no
Details on sampling:
Three series of the reference solutions were measured with two parallel injections each. Three series of the test item solution were measured with two parallel injections each. The retention times were determined. The three calibration series and three test item series were measured alternately.
Details on test conditions:
Test Solutions:
The reference items were dissolved in methanol. The concentrations of the stock solutions were about 1 mg/mL.
These stock solutions were diluted with eluent resulting in concentrations of the test solutions of approximately 10 μg/mL. The sodium nitrate and DDT stock solutions were diluted with eluent resulting in concentration of the test solution of approximately 100 and 50 μg/mL.
The test item was dissolved in methanol. The concentration of this solution was about 1 mg/mL. It was diluted with eluent to 10 μg/mL.
Key result
Type:
log Koc
Value:
4.2 dimensionless
Temp.:
25 °C
Type:
Koc
Value:
15 968 dimensionless
Temp.:
25 °C
Details on results (HPLC method):
- Retention times (mean value) of reference substances used for calibration:
sodium nitrate (1.69 minutes), acetanilide (3.24 minutes), methyl benzoate (3.67 minutes), isoproturon (3.78 minutes), linuron (4.77 minutes), naphthalene (5.23 minutes), fenthion (6.89 minutes), DDT (13.35 minutes).
- Details of fitted regression line (log k' vs. log Koc): log k = 0.205 * log Koc - 0.274 (Rsq = 0.980)
- Average retention data for test substance: 8.25 min
Validity criteria fulfilled:
yes
Conclusions:
Using the HPLC method the log Koc of 1,1'-(1,1,2,2-tetramethylethylene)dibenzene was determined to be 4.20 at 25 °C.
Executive summary:

The adsorption coefficient (Koc) on soil and sewage sludge of 1,1'-(1,1,2,2-tetramethylethylene)dibenzene was assessed according to EU method C.19 and OECD guideline no. 121 using the HPLC method. Based on the retention time measured for 1,1'-(1,1,2,2-tetramethylethylene)dibenzene (8.25 min), the log Koc was estimated to be 4.20 ± 0.007 (95 % confidence interval) corresponding to a Koc of 15968 ± 254.8 (95 % confidence interval) at 25 °C.

Description of key information

 The Koc of the test item was determine using the HPLC method to be 15968 ± 254.8 (95 % confidence interval) and the log Koc to be 4.2 at 25 °C, respectively.

Key value for chemical safety assessment

Koc at 20 °C:
15 968

Additional information

The adsorption coefficient (Koc) on soil and sewage sludge of 1,1'-(1,1,2,2-tetramethylethylene)dibenzene was assessed according to EU method C.19 and OECD guideline no. 121 using the HPLC method. Based on the retention time measured for 1,1'-(1,1,2,2-tetramethylethylene)dibenzene (8.25 min), the log Koc was estimated to be 4.20 ± 0.007 (95 % confidence interval) corresponding to a Koc of 15968 ± 254.8 (95 % confidence interval) at 25 °C.