Potassium mercaptoacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05-June-2000 - 19-July-2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Potassium mercaptoacetate

EC Number:

252-038-4

EC Name:

Potassium mercaptoacetate

Cas Number:

34452-51-2

Molecular formula:

C2H4O2S.K

IUPAC Name:

potassium sulfanylacetate

Constituent 2

Reference substance name:

KTG

IUPAC Name:

KTG

Test material form:

solid - liquid: suspension

Details on test material:

Test substance: aqueous solution of potassium thioglycolate
Batch number: B00S5611
Content of active ingredient (potassium thioglycolate): 42.5 % (w/w)

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

- Male and female Sprague Dawley rats CD (Crl: CD (SD) IGS BR)
- Supplier: Charles River (UK) Ltd, Margate, Kent, UK
- Weight: 221-229 g (M), 202-232 g (F)
- Age: 12 weeks
- Acclimatization:  at least 5 days
- Housing: groups of 3 by sex in solid-floor polypropylene cages
- Diet: ad libitum except fasting (Rat and Mouse Expanded Diet N°1, Special  Diets Services Limited, Witham, Essex, UK)
- Water: drinking water ad libitum
- Fasting: overnight before dosing and 3-4 hours after dosing

CONDITIONS:
- Temperature: 19-25 °C
- Relative Humidity: 30-70 %
- Air changes: approx. 15/hour
- Dark-Light cycle (hours): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

200, 500 and 2000 mg/kg potassium thioglycolate as 42.5% solution.

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

All animals were dosed once only by gavage. The animals were observed for  death or overt signs of toxicity 1/2, 1, 2 and 4 hours after dosing and  subsequently twice daily for up to 14 days. Individual body weights were  recorded prior to dosing and 7 and 14 days after treatment or at death. At the end of the observation period, animals were subjected to  macroscopic examination. No tissue was retained.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

>= 200 - <= 500 mg/kg bw

Based on:

test mat.

Mortality:

All animals treated with 2000 mg/kg, one animal treated with 500  mg/kg and one animal treated with 200  mg/kg were found dead during the day of dosing. The two remaining animals treated with 500 mg/kg were found dead one day after dosing.

Clinical signs:

other: Hunched posture was commonly noted at dose levels of 2000 mg/kg, 500 mg/kg and in females treated with 200 mg/kg with lethargy also commonly noted at 2000 mg/kg and ataxia also commonly noted at 500 mg/kg. lncidents of decreased respiratory rate and labou

Gross pathology:

Abnormalities noted at necropsy of animals that died during the study  were haemorrhagic lungs, dark liver, dark kidneys, epithelial sloughing of thegastric mucosa and haemorrhage of the bladder. No abnormalities were noted at necropsy of animals that were killed at the end of the study.

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

The acute oral median lethal dose LD50 of potassium thioglycolate 43% solution was estimated to be in the range of 200-500 mg/kg.