Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-616-2 | CAS number: 123-30-8
- Measured Concentrations: The test concentrations were measured at the start and the end of the test using HPLC.
*: Arithmetic mean of measured concentrations - Water chemistry (pH, DO and temperature in test): Water chemistry and temperature were measured for each concentration at least once a day. pH: 7.3 - 7.6 DO: 7.9 - 8.3 mg/L (more than 60% of saturation) Water Temperature: 23.0 - 24.2 C -Effect Data(mortality): LC50 (96hr) 0. 82 mg/L (mc) 95% Confidence interval: 0.780 - 1.11 mg/L NOEC (96hr) 0.526 mg/L (mc) mc: based on measured concentrations - Cumulative Mortality: None of test organisms were killed during exposure period at control, 0.243, 0.339 and 0.526 mg/L.
-Other Effect: As toxic symptoms, abnormal swimming behaviour and immobility were observed during test period in concentrations of 0.526, 0.809 and 1.13 mg/L. No symptoms were observed in control, 0.243 and 0.339 mg/L. Appearance of test solutions in concentrations of 1.13 and 0.809 mg/L were slightly brown and transparent, while that of other concentrations were colourless and transparent.
Effect of test item on survival of larvae of Zebra Fish: Experimental part A (parent molecule)
Effect of test item on survival of larvae of Zebra Fish: Experimental part B (degradation products):
Validity criteria: overall survival of embryos in the negative (dilution-water) control was ≥ 90% until the end of the 96 hrs exposure; Hatching rate in the negative control was ≥ 80% at the end of 96 hrs exposure; The water temperature in the water bath was constantly 26°C.
The purpose of this non GLP study based on OECD 236, was to determine the effects of the test item (parent molecule) and its possible degradation products on the development of embryos of zebra fish (Danio rerio). In order to compare the toxic potentials, the study comprised two experimental parts: In Experimental Part A, the parent molecule of the test item was tested. In Experimental Part B, the degradation products of the test item were tested. For Experimental Part A, a semi-static test design with test medium renewal after 24 hours was applied. Experimental Part B was performed static.
The exposure periods of both experimental parts were run in parallel. The duration of the test was 96 hours. For each treatment, 20 eggs were introduced into the test for each of the two experimental parts. The nominal test item concentrations for both experimental parts were 0.10, 1.0, 10, and 100 mg/L. For each experimental part, a control (test water without test item) was run in parallel. The eggs/embryos were observed daily for coagulated embryos, lack of somite formation, non detachment of the tail, mortality and hatching of larvae. The appearance of the test media were recorded daily. No analytical work was performed.
For experimental part A (parent molecule), LC50 -96H=0.15 mg/L, LC100=1.0 mg/L, NOEC=0.10 mg/L, LOEC=1.0 mg/L.
For experimental part B (degradation products), LC50 -96H=1.4 mg/L, LC100=10 mg/L, NOEC=1.0 mg/L, LOEC=10 mg/L.
The results obtained during these 96-hour toxicity tests indicate, that the parent molecule of the test item is more toxic to larvae of zebra fish compared to its degradation products.
The short-term 96 hr LC50 for fish, based on the mean of measured concentrations, was 0.82 mg/L in Oryzias latipes as reported in the key study. The NOEC value was 0.526mg/L.
In addition to the acute toxicity study a fish embryo acute toxicity (FET) study was conducted on both the parent and degradation product. The results of this study found that the parent product was more toxic than the degradation products.
parent molecule, LC50 -96H=0.15 mg/L, LC100=1.0 mg/L, NOEC=0.10 mg/L, LOEC=1.0 mg/L
degradation products, LC50 -96H=1.4 mg/L, LC100=10 mg/L, NOEC=1.0 mg/L, LOEC=10 mg/L.
The key study is an Acute Toxicity in Oryzias latipes completed in 1997 based on OECD method 203 and GLP with a reported LC50 based on mean of measured concentration of LC50 of 0.82 mg/L. Concentrations of PAP maintained between 56.6 and 61.5 % depending on the nominal concentration.Values based on mean measured concentrations calculated directly from data in the study report.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again