6,15-dihydroanthrazine-5,9,14,18-tetrone

Endpoint summary

Additional information

Short-term toxicity to fish

The acute toxicity to fish was examined in a guideline study (BASF AG, 1991) following German national standard DIN 38412, part L15 (1982) with the surrogate test substance (CAS 1324-29-4) on the species Leuciscus idus. The test fish were exposed to the substance for 96 hours in a static test system. At test termination a LC50 > 10000 mg/L (nominal) was determined.

 

Long-term toxicity to fish

In accordance to column 2 of REACh Annex IX, long-term toxicity testing shall be proposed by the registrant, if the chemical safety assessment according to Annex I indicates the need to investigate further the effects on aquatic organisms. The choice of the appropriate test(s) depends on the results of the chemical safety assessment. According to Annex I of this regulation, the chemical safety assessment triggers further action when the substance or the preparation meets the criteria for classification as dangerous according to Directive 67/548/EEC or Directive 1999/45/EC or is assessed to be a PBT or vPvB. The hazard assessment of the substance reveals neither a need to classify the substance as dangerous to the environment, nor is it a PBT or vPvB substance, nor are there any further indications that the substance may be hazardous to the environment. Based on this assessment and for reasons of animal welfare a long-term toxicity testing on fish is not provided.

 

Short-term toxicity to aquatic invertebrates

The acute toxicity of the test substance to aquatic invertebrates was examined in a GLP guideline study (BASF SE 2012) according to OECD 202 (2004) with the test species Daphnia magna in a static test system. A saturated solution of the test substance prepared at a loading concentration of 1 mg/L had no observable effect on Daphnia magna in the test media and under test conditions. Thus, an EC50 > 1 mg/L (nominal) after the 48 hour exposure period could be determined.

 

Long-term toxicity to aquatic invertebrates

In accordance to column 2 of REACh Annex IX, long-term toxicity testing shall be proposed by the registrant, if the chemical safety assessment according to Annex I indicates the need to investigate further the effects on aquatic organisms. The choice of the appropriate test(s) depends on the results of the chemical safety assessment. According to Annex I of this regulation, the chemical safety assessment triggers further action when the substance or the preparation meets the criteria for classification as dangerous according to Directive 67/548/EEC or Directive 1999/45/EC or is assessed to be a PBT or vPvB. The hazard assessment of the substance reveals neither a need to classify the substance as dangerous to the environment, nor is it a PBT or vPvB substance, nor are there any further indications that the substance may be hazardous to the environment. Based on this assessment and for reasons of animal welfare a long-term toxicity testing on aquatic invertebrates is not provided.

 

Toxicity to algae and cyanobacteria

The toxicity of the test substance to aquatic algae was investigated in a GLP guideline study according to OECD 201 (2011) with Desmodesmus subspicatus (BASF SE 2012). The substance had no observable inhibitory effect on algae growth up to its saturation limit in the test media and under test conditions. The ErC50 value was determined to be > 1 mg/L (nominal).

 

Toxicity to microorganisms

The respiration inhibition of the surrogate test substance (CAS 1324-29-4) to the bacteria Pseudomonas putida was investigated in a guideline study (BASF AG 1991) following German standard DIN 38412, part 27 (1992). After a 30 min exposure period, an EC10 of 250 mg/L (nominal) was determined.