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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: National guideline study with acceptable restrictions

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1986
Reference Type:
other: national guideline
Title:
Unnamed
Year:
1980
Reference Type:
publication
Title:
Aquatic toxicological aspects of dithiocarbamates and related compounds. Short-term tests
Author:
Van Leeuwen CJ, Maas-Diepeveen JL, Niebeek G, Vergouw WHA, Griffioen OPS, Luyken MW
Year:
1985
Bibliographic source:
Aquat. Toxicol. 7, 145-164
Reference Type:
publication
Title:
A simplified method of evaluating dose-effect experiments
Author:
Litchfield JT Jr, Wilcoxon F
Year:
1949
Bibliographic source:
J. Pharmacol. Exp. Therap. 96, 99-113

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: NEN 6501 (1980) (Dutch Standardization Organization)
Deviations:
yes
Remarks:
: only "slight modifications (Van Leeuwen et al. 1985)"; The daphnids were fed on 1E8 cells/L Chlorella pyrenoidosa
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-chloroaniline
EC Number:
202-426-4
EC Name:
2-chloroaniline
Cas Number:
95-51-2
Molecular formula:
C6H6ClN
IUPAC Name:
2-chloroaniline
Details on test material:
purity >/= 99%

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
isopropanol (purity 99%)

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.13 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CL: 0.09-0.19 mg/L
Reported statistics and error estimates:
LC50 values and their 95% confidence intervals were calculated according to Litchfield and Wilcoxon (1949)

Any other information on results incl. tables

no further data

Applicant's summary and conclusion

Executive summary:

Maas-Diepeveen and van Leeuwen (1986)

The EC50 (48 h) for Daphnia magna was determined in a laboratory study according to the Dutch national guideline NEN 6501 of 1980 (Dutch Standardization Organization) to be 0.13 mg/L (95% CV: 0.09 -0.19 mg/L), based on test material.