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Administrative data

Description of key information

Key value for chemical safety assessment

Skin sensitisation

Endpoint conclusion
Endpoint conclusion:
no adverse effect observed (not sensitising)
Additional information:

Following a preliminary screening test in which no clinical signs of toxicity were noted at a concentration of 100% dibromomethane, this concentration was selected as the highest dose investigated in order to assess the skin sensitisation potential of the test material in the CBA/Ca strain mice.

Three groups, each of five animals, were treated by topical application to the dorsal surface of the ear with 25 µl undiluted test material (99.5% purity, dibromomethane) or the test material as a solution in acetone/olive oil 4:1 at concentrations of 50% or 25% v/v. A further group of five animals was treated with acetone/olive oil 4:1 alone.

Results: The Stimulation Index (SI) of the vehicle control group were 1.39, 0.63 and 0.70 for all concentrations of the test material investigated (100%, 50% & 25%, respectively). The positive control group (a concentration of 15% of α-hexylcinnamaldehyde in 4:1 Acetone/Olive oil) showed an acceptable response for a valid study with an SI of 4.10. Hence, it was concluded that the DBM is not skin sensitizer. 


Migrated from Short description of key information:
Test/Control < 3 for all concentrations of the test material investigated (100%, 50% & 25%). The stimulation indexes (SI's) were 1.39, 0.63 and 0.70 respectively. The test material is a non sensitiser.

The positive control group (a concentration of 15% of alpha-hexylcinnamaldehyde in 4:1 Acetone/Olive oil) showed an acceptable response for a valid study with an SI of 4.10.

Justification for selection of skin sensitisation endpoint:
only study available.

Respiratory sensitisation

Endpoint conclusion
Additional information:

Not sensitizing

Justification for classification or non-classification