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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted prior to availability of the appropriate OECD and OPPTS testing guidelines. However, the study was well documented and is scientifically acceptable. Hence, it was identified as a key study and was graded klimisch 2.
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1979
Report date:
1979

Materials and methods

Principles of method if other than guideline:
Rabbits were inoculated with DBM in the eye. Examinations for signs of irritation or injury were made at intervals of 1, 2, 4, 8, 24, 48 and 72 hours after the rabbits were exposed to the test material. Scoring was performed according to Draize procedure.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Dibromomethane
EC Number:
200-824-2
EC Name:
Dibromomethane
Cas Number:
74-95-3
Molecular formula:
CH2Br2
IUPAC Name:
dibromomethane

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White
Details on test animals or tissues and environmental conditions:
No further information

Test system

Controls:
other: The contralateral eye served as control.
Amount / concentration applied:
0.1 ml (3 drops) of the test material (unspecified purity, DBM) in the eye.
Observation period (in vivo):
Examinations for signs of irritation or injury were made at intervals of 1, 2, 4, 8, 24, 48 and 72 hours after the rabbits were exposed to the test material.
Number of animals or in vitro replicates:
Six New Zealand White (NZW) rabbits
Details on study design:
Six New Zealand White (NZW) rabbits were inoculated with 0.1 ml (3 drops) of the test material (unspecified purity, DBM) in the eye. The tested rabbits were free of any pre-test ocular abnormalities. The contralateral eye served as control. Examinations for signs of irritation or injury were made at intervals of 1, 2, 4, 8, 24, 48 and 72 hours after the rabbits were exposed to the test material. Scoring was performed according to Draize procedure.

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 72 hours
Remarks on result:
other: moderate eye irritation
Irritant / corrosive response data:
Immediately following the introduction of DBM into the conjunctive sac, the eye closed and the rabbit rubbed the eye with a foot. After several minutes the rubbing stopped. Within 8 hours a capillary congestion and edema of the third eyelid developed.

Applicant's summary and conclusion

Interpretation of results:
moderately irritating
Remarks:
Migrated information
Conclusions:
DBM caused moderate eye irritation which lasted 3 days or more following inoculation.
Executive summary:

Six New Zealand White (NZW) rabbits were inoculated with 0.1 ml (3 drops) of the test material (unspecified purity, DBM) in the eye. The tested rabbits were free of any pre-test ocular abnormalities. The contralateral eye served as control. Examinations for signs of irritation or injury were made at intervals of 1, 2, 4, 8, 24, 48 and 72 hours after the rabbits were exposed to the test material. Scoring was performed according to Draize procedure.

Immediately following the introduction of DBM into the conjunctive sac, the eye closed and the rabbit rubbed the eye with a foot. After several minutes the rubbing stopped. Within 8 hours a capillary congestion and edema of the third eyelid developed. DBM caused moderate eye irritation which lasted 3 days or more following inoculation.