N,N''-hexane-1,6-diylbis[N'-benzylurea]

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

N,N''-hexane-1,6-diylbis[N'-benzylurea]

EC Number:

254-274-3

EC Name:

N,N''-hexane-1,6-diylbis[N'-benzylurea]

Cas Number:

39072-70-3

Molecular formula:

C22H30N4O2

IUPAC Name:

N',N'''-hexane-1,6-diylbis(1-benzylurea)

Details on test material:

Description White powder (determined at NOTOX)
Batch 259-635
Purity > 90%

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Frequency at t=0 h and t=48 h
Volume 2 ml from the approximate centre of the test vessels
Storage Samples were stored in a freezer until analysis.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Hardness:

180 mg/l as CaCO3

Test temperature:

19.7 - 20.8°C

pH:

7.8

Dissolved oxygen:

8.9 - 9.8

Nominal and measured concentrations:

Analysis of the samples taken from the filtrates prepared at 1.0 and 100 mg/l showed that measured concentrations remained fairly stable over the test period. Analyses of the filtrate prepared at 1.0 mg/l showed measured concentrations of 11.0 and 12.1 µg/l at t=0 and 48 hours. Analyses of the filtrate prepared at 100 mg/l showed measured concentrations of 17.1 and 19.6 µg/l at t=0 and 48 hours, respectively. The average measured concentrations were calculated to correspond to 12 and 18 µg/l, for the filtrates prepared at 1.0 and 100 mg/l, respectively.

Details on test conditions:

Test vessels 100 ml, all-glass
Medium Adjusted ISO medium
Number of daphnids 20 per concentration
Loading 5 per vessel containing 80 ml of test solution
Light 16 hours photoperiod daily
Feeding No feeding
Aeration No aeration of the test solutions.
Introduction of daphnids Within 1 hour after preparation of the test solutions.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 100 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The 48h-EC50 exceeded 18 µg/l, being the average measured concentration in a 0.45 µm filtered solution prepared at a loading rate of 100 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes