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Toxicological information

Dermal absorption

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Administrative data

Endpoint:
dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: no guideline study
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1984
Report date:
1984
Reference Type:
publication
Title:
Fragrance material review on methyl ionone (mixture of isomers)
Author:
J. Lalko, A. Lapczynski , D. McGinty, S. Bhatia, C.S. Letizia, A.M. Api
Year:
2007
Bibliographic source:
Food and Chemical Toxicology 45: S300–S307

Materials and methods

Test material

Constituent 1
Chemical structure
Reference substance name:
Ionone, methyl-
EC Number:
215-635-0
EC Name:
Ionone, methyl-
Cas Number:
1335-46-2
Molecular formula:
C14H22O
IUPAC Name:
.
Details on test material:
Test material was a mixture of 14C labeled and non-radioactive material dissolved in ethanol at a concentration of 10%.
Radiolabelling:
yes
Remarks:
C14

Test animals

Species:
rat
Strain:
other: naked

Administration / exposure

Vehicle:
ethanol
Duration of exposure:
1, 6 or 16 hours
Doses:
10% in ethanol

Results and discussion

Any other information on results incl. tables

600 µg/cm2: skin absorption

After a 1 hour contact with naked rat skin approximately 74% of the test material applied was recovered with 308.76 ug/cm2 found on the skin surface, 27.11 ug/cm2 found in the horny layer of the skin, 107.13 ug/cm2 found in the remaining skin tissue layers, and 0.04 ug/cm2 found in the chamber liquid.

After 6 hours contact with naked rat skin approximately 74% of the test material applied was recovered with 195.17 ug/cm2 found on the skin surface, 21.56 ug/cm2 found in the horny layer of the skin, 219.94 ug/cm2 found in the remaining skin tissue layers, and 3.96 ug/cm2 found in the chamber liquid.

After 16 hours contact with naked rat skin approximately 60% of the test material applied was recovered with 70.35 ug/cm2 found on the skin surface, 32.27 ug/cm2 found in the horny layer of the skin, 249.75 ug/cm2 found in the remaining skin tissue layers, and 7.48 ug/cm2 found in the chamber liquid.

The unrecovered test material was presumably lost by evaporation from the skin surface.

   recovery [%]

 skin surface  
[µg/cm²]

 horny layer  

[µg/cm²]

 remaining skin tissue layers [µg/cm²]

 chamber liquid  

[µg/cm²]

 1 hour

 74

 308.76

 27.11

 107.13

 0.04

 6 hours

 74

 195.17

 21.56

 219.94

 3.96

 16 hours

 60

 70.35

 32.27

 249.75

 7.48

Applicant's summary and conclusion