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Toxicity to microorganisms

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Description of key information

The inhibition of the degradation activity of activated sludge is not anitcipated when introduced  in appropriate low concentrations. 

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The respiration inhibition induced by methylionone in domestic activated sludge was investigated in a guideline study according to OECD 209. After an inoculation time of 30 minutes the test was terminated and an EC50>1000 mg/l was determined (BASF AG, 1996). In a second test investigating the growth inhibition in Pseudomonas putida as induced by methylionone an EC50 of 6640 mg/l (EC50=1000 mg/l) was detected after 16 hours of exposure (BASF AG, 1996).