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EC number: 213-110-0 | CAS number: 924-88-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 1999 to July 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.4 (Acute Toxicity: Dermal Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Diisopropyl succinate
- EC Number:
- 213-110-0
- EC Name:
- Diisopropyl succinate
- Cas Number:
- 924-88-9
- Molecular formula:
- C10H18O4
- IUPAC Name:
- diisopropyl succinate
- Details on test material:
- Name "DIISOPROPYLSUCCINAT".
Chemical name Succinic acid diisopropyl ester.
Trade name DIPS.
CAS No. 924-88-9.
Batch No. EMBS 302.
Purity At least 99 %.
Density 0.9847.
Kp 247 °C.
Solubility in water Insoluble.
Storage In the refrigerator, in the dark.
Date of expiry June 1999.
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- Species and strain: Rabbits, New Zealand White.
Supplier: J. Schitkovits, A-2620 Natschbach.
Number and sex: 3 females.
Body weight (at the start and at the termination of the study):
Animal No. 41: 2.6 kg and 2.8 kg.
Animal No. 42: 2.6 kg and 2.8 kg.
Animal No. 43: 2.3 kg and 2.4 kg.
Hygiene: Optimal hygienic conditions.
Room number: EH1-17.
Room temperature: Average of 20 °C.
Relative humidity: Average of 52 %.
Air exchange: 12 per hour.
Light: Artificial light from 6 a.m. to 6 p.m.
Cages: Individual caging in metal wire cages, Ehret GmbH, D-79312 Emmendingen, type KK 016R, 79 cm x 59 cm x 35 cm.
Feed: Altromin 2023 diet for rabbits, ad libitum. Random samples of the feed are analysed for contaminants by Altromin, D-32791 Lage.
Water: Tap water from an automatic watering system, ad libitum.
Identification: Labelling with felt-tipped pen in a pinna.
Acclimatisation: 12 days.
Test system
- Type of coverage:
- semiocclusive
- Preparation of test site:
- other: clipped
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.5 mL per animal
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 1, 24, 48 and 72 hours after patch removal.
- Number of animals:
- 3
- Details on study design:
- Hair was clipped on the dorsal area of the trunk one day before the application. An electric hair clipper, Aesculap GH 204, with a 1 mm cutterhead was used.
The test sites were located on the dorsal thoracal region.
Samples of 0.5 ml of the test substance were spread on cellulose patches 1) in a size of about 2.5 cm x 2.5 cm and were applied to the test sites. They were held in place by fixing them marginally with non irritating tapes 2). The application sites were covered semi-occlusively by a dressing 3).
Access by the animal to the application sites was prevented by a plastic collar.
The duration of exposure was 4 hours.
At the end of the exposure period the dressing, the tapes with the patches and the collar were removed, residual test substance was removed with wet Kleenex-tissues, if necessary.
1) Pur Zellin-Tupfer, obtained by Fa. Hartmann , A-2355 Wiener Neudorf.
2) "Blenderm ®" surgical tape, hypoallergenic, 3M, Medical Products Division, St. Paul, MN 551444, USA.
3) Self adhesive non woven fabric, hypoallergenic, made by Beiersdorf AG, D-20245 Hamburg.
The treated areas and the surrounding untreated skin (control area) of the animals were examined for erythema/eschar and oedema as well as for other local alterations approximately 1, 24, 48 and 72 hours after patch removal.
No examinations were performed thereafter.
The scores noted, 24, 48 and 72 h after the patch removal were decisive for the interpretation of the results and the conclusion.
The skin was examined using a cold light source KL 1500 electronic.
Dermal irritation was described and recorded according to the scoring scheme for acute dermal irritation/corrosion tests.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 1, 24, 48, 72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- other: not applicable
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 1, 24, 48, 72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- other: not applicable
- Irritant / corrosive response data:
- All areas to be treated with the test substance and all control areas were normal before the application and at each observation time.
- Other effects:
- None
Any other information on results incl. tables
|
Mean Scores for Animal No.: |
||
|
41 |
42 |
43 |
Erythema / Eschar: |
0.0 |
0.0 |
0.0 |
Oedema: |
0.0 |
0.0 |
0.0 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- As all areas treated with the test substance were normal before the application and at each observation time the test substance is not irritating to the skin.
- Executive summary:
The aim of this study was to investigate possible irritation or corrosion by the test material following a single application to the intact skin of rabbits.
Methods
Methods and investigations were performed in conformance with the OECD Guideline 404 and the Directive 92/69/EEC, method B.4.
Administration of the test substance
0.5 ml"DIISOPROPYLSUCCINAT"was applied via a patch to a site of about 2.5 cm x 2.5 cm of the intact skin of each of 3 rabbits and covered by semi-occlusive dressing.
The duration of the exposure was 4 hours.
Investigations
· Body weights: at the start and at the termination of the test.
· General signs of toxicity: once daily.
· Skin examination: 1, 24, 48 and 72 h after patch removal.
Results
General signs of toxicity
No general toxic effects were observed.
Skin examination
All exposed skin sites were normal at each examination term.
Conclusion
The following mean scores were calculated for each animal from the individual examinations performed 24 h, 48 h and 72 h p.a.:
Mean Scores for Animal No.:
41
42
43
Erythema / Eschar:
0.0
0.0
0.0
Oedema:
0.0
0.0
0.0
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