Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
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EC number: 265-086-6 | CAS number: 64741-84-0 A complex combination of hydrocarbons obtained as the raffinate from a solvent extraction process. It consists predominantly of aliphatic hydrocarbons having carbon numbers predominantly in the range of C5 through C11 and boiling in the range of approximately 35°C to 190°C (95°F to 374°F).
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP, non-guideline, published , adequate for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 995
Materials and methods
- Principles of method if other than guideline:
- Method as described by Galloway S et al (1987) Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: evaluations of 108 chemicals Environ Mol. Mutagen, Vol. 10, pp. 1-175.
- GLP compliance:
- yes
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- Isoprene
- EC Number:
- 201-143-3
- EC Name:
- Isoprene
- Cas Number:
- 78-79-5
- Molecular formula:
- C5H8
- IUPAC Name:
- 2-methylbuta-1,3-diene
- Details on test material:
- purity > 99%.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Chinese hamster ovary (CHO) cells
- Test concentrations with justification for top dose:
- 1600, 3000, 5000 µg/mL
Results and discussion
Test results
- Genotoxicity:
- negative
- Remarks on result:
- other: other: Chinese hamster ovary (CHO) cells
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
No increases in chromosomal aberrations were noted in cultured CHO cells treated with isoprene, with or without S9.
Applicant's summary and conclusion
- Conclusions:
- Isoprene did not induce chromosomal aberrations in vitro in cultures of Chinese hamster ovary cells.
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