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Diss Factsheets
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EC number: 202-425-9 | CAS number: 95-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: US EPA (1971): Algal Assay Procedure - Bottle Test. National Eutrophication Research Program, Corvallis, 82 ppg
- Deviations:
- yes
- Remarks:
- Test vessels were modified according to Galassi and Vighi (1981) in order to maintain constant concentration and to allow the sampling of the culture medium without opening the vessel
- Principles of method if other than guideline:
- Additionally to the growth inhibition test, a physiological test was carried out in order to determine the EC50 (3 h) on the efficiency of photosynthesis measuring the 14C uptake. A Selenastrum algal biomass, corresponding to 0.1 mg dry weight was inoculated into light and dark bottles (100 mL) containing AAPBT medium and different concentrations of the test material in three replicates each. The samples were incubated for 3 h after addition of 1 mL 14C-NaHCO3 solution. At the end of the incubation the contents of each bottle were filtered through 0.45 µm membrane filters and the radioactivity efficiency was calculated by the channels ratio method. The assimilation coefficients (photosynthesis/unit biomas) were calculated as disintegration per minute (counts/min mg algal dry weight).
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Vehicle:
- not specified
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- not specified
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC0
- Effect conc.:
- < 0.88 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- not specified
- Basis for effect:
- growth rate
- Duration:
- 96 h
- Dose descriptor:
- EC100
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- not specified
- Basis for effect:
- growth rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- other: photosynthesis inhibition
- Conclusions:
- The EC50 (96 h) for growth inhibition of Selenastrum capricornutum (now: Pseudokirchneriella subcapitata) was determined in a laboratory study using the Algal Assay Procedure - Bottle Test (US EPA, 1971) to be 2.2 mg/L. Furthermore, the EC0 (96 h) could not be determined (< 0.88 mg/L) and the EC100 (96 h) was 17.0 mg/L.
Additionally, the EC50 (3 h) for photosynthesis inhibition was reported to be 10.0 mg/L. - Executive summary:
Calamari et al (1983).
The EC50 (96 h) for growth inhibition of Selenastrum capricornutum (now: Pseudokirchneriella subcapitata) was determined in a laboratory study using the Algal Assay Procedure - Bottle Test (US EPA, 1971) to be 2.2 mg/L. Furthermore, the EC0 (96 h) could not be determined (< 0.88 mg/L) and the EC100 (96 h) was 17.0 mg/L.
Additionally, the EC50 (3 h) for photosynthesis inhibition was reported to be 10.0 mg/L.
Reference
Description of key information
The 96h EC50 for growth inhibition of Selenastrum capricornutum (now: Pseudokirchneriella subcapitata) was determined in a laboratory study by Calamari et al (1983) using the Algal Assay Procedure - Bottle Test (US EPA, 1971). The 96h EC50 was determined to be 2.2 mg/L, however a NOEC (96 h) could not be determined (< 0.88 mg/L). The EC100 (96 h) was determined as 17.0 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2.2 mg/L
Additional information
The 96 h EC50 for growth inhibition of Selenastrum capricornutum (now: Pseudokirchneriella subcapitata) was determined in a laboratory study by Calamari et al (1983) using the Algal Assay Procedure - Bottle Test (US EPA, 1971). The 96 h EC50 was determined to be 2.2 mg/L, however a NOEC (96 h) could not be determined (< 0.88 mg/L). The EC100 (96 h) was determined at 17.0 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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