Registration Dossier

Ecotoxicological information

Toxicity to terrestrial plants

Currently viewing:

Administrative data

Endpoint:
toxicity to terrestrial plants: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
August 1990
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Guideline:
other:
Principles of method if other than guideline:
Two fractionation schemes were developed for analyzing RDX in plant tissues.
The initial step of both methods was solvent extraction of RDX from acid-hydrolyzed plant tissue. The methods differed only in the fractionation of the extracted plant components. It was necessary to further fractionate the organic extracts to remove plant pigments, which would interfere with subsequernt HPLC analysis. One method was based or. fractionation using a strong-cation exchange resin, whereas the other fractionation was based on Florisil chromatography.
The initial solvent extraction of plant leaves (up to the Florisil chromatography step) was identical for both methods.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Test type:
early seedling growth toxicity test
Study type:
laboratory study
Substrate type:
natural soil

Results and discussion

Effect concentrationsopen allclose all
Species:
Phaseolus vulgaris
Duration:
60 d
Dose descriptor:
other: tissue concentration
Effect conc.:
>= 216.7 - <= 602.6 other: µg RDX Equivalents/g Fresh Weight
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Triticum aestivum
Duration:
60 d
Dose descriptor:
other: tissue concentration
Effect conc.:
>= 75.7 - <= 549.9 other: µg RDX Equivalents/g Fresh Weight
Conc. based on:
test mat.
Basis for effect:
growth
Species:
other: Bromus mollis
Duration:
60 d
Dose descriptor:
other: tissue concentration
Effect conc.:
>= 43.8 - <= 564.5 other: µg RDX Equivalents/g Fresh Weight
Conc. based on:
test mat.
Basis for effect:
growth

Applicant's summary and conclusion